Examples of 'dialysis buffer' in a sentence
Meaning of "dialysis buffer"
dialysis buffer: A solution used in dialysis procedures to maintain the appropriate chemical environment for separating molecules based on size. Example: 'The dialysis buffer helped in purifying the protein sample.'
How to use "dialysis buffer" in a sentence
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dialysis buffer
The dialysis buffer is changed twice.
The column is equilibrated with the dialysis buffer solution described above.
The dialysis buffer was as described above.
The dialysate was loaded onto a MonoQ column which was equilibrated with the same dialysis buffer.
Dialysis buffer samples were used to subtract background signal.
The column was equilibrated with the above-described dialysis buffer.
The dialysis buffer being changed twice.
Each cell pellet was washed with another 40 ml of dialysis buffer as detailed above.
The final dialysis buffer was used as reference and subtracted from the scans.
Normally, the conductivity of the sample is monitored against the dialysis buffer.
The dialysis buffer was used as reference in the DSC scans.
Finally, any remaining reagents may be removed by dialysis using a final dialysis buffer.
The dialysis buffer was changed every 8 hours.
Check protein concentration of the elutions and dialyse overnight at 4°C in dialysis buffer.
During dialysis buffer was changed 3 times.
See also
The sample was eluted in 1 mL of Dialysis Buffer.
Dialysis buffer was exchanged once and dialysis continued for another 2 h.
A dilution of 25µg/ml was prepared with a millipored dialysis buffer.
The dialysis buffer was changed twice before collecting the morphine-BSA conjugate.
Each cell pellet was resuspended in 40 ml of dialysis buffer as detailed above.
The dialysis buffer was changed to phosphate-buffered saline and the dialysis continued.
Dialysis was continued in a fresh liter of dialysis buffer for 2-4 hours the next morning.
The dialysis buffer is then changed to 5mM EDTA / PBS and dialyzed for another two hours.
The dialysis was performed against a 200-fold volume of dialysis buffer in dissolution flask.
The dialysis buffer was used as reference in Differential Scanning Calorimetry ( DSC ).
As controls, the HBcAg-JUN and the hGH-FOS solutions were also dialyzed against dialysis buffer.
Dialysis buffer was replaced 3 times over a 2-3 day period at which point monomerization is also completed.
The elution is performed by a gradient consisting of the dialysis buffer but containing 0.5M of NaCl.
After 24 hours the dialysis buffer is changed and the virosome solution dialyzed for a further 24 hours.
The dialysis was carried out over 24 hours at 4°C with three changes of dialysis buffer.
Prepare additional dialysis buffer as determined in step 1, but omit DTT.
Dialyzed the viral suspension samples against 1800 mL of the dialysis buffer 4 °C for overnight.
Furthermore, the dialysis buffer for GImU additionally contained 10 mM B-mercaptoethanol.
The sample was resuspended in 100ml dialysis buffer and dialyed in the buffer for 2 X 2 hours.
The dialysis buffer was isotonic buffered saline pH 7.4.
In one preferred embodiment, the dialysis buffer is 15 mM HEPES containing 5 % dextrose at approximately pH 7.
Dialysis buffer ( closed squares ).
At 22 ° C. The dialysis buffer was exchanged twice per day until the end of the experiment.
The dialysis buffer was changed and dialysis continued at 5°C for another 72 hours.
The dialysis buffer was changed every 4-6 hours, and a total of 6 L of dialysis buffer was used.
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Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much