Examples of 'diaminobenzidine' in a sentence
Meaning of "diaminobenzidine"
Diaminobenzidine is a chemical compound commonly used in immunohistochemistry to visualize proteins and other antigens in cells and tissues
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- Any of several isomeric diamino derivatives of benzidine
How to use "diaminobenzidine" in a sentence
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diaminobenzidine
Finally a diaminobenzidine chromogenic substrate system is used.
The slides were developed with diaminobenzidine solution.
Diaminobenzidine substrate was then prepared and added to the membrane.
Suitable chromogenic dyes include diaminobenzidine.
Diaminobenzidine was used as a chromogen.
The reaction product was developed using the chromogen diaminobenzidine.
Diaminobenzidine served as our chromogen and color development followed by hematoxylin counterstaining and mounting.
Immunoreactivities were visualized with diaminobenzidine.
Diaminobenzidine plus a chromogen-substrate was used to develop antibody fixation.
The colour reactions of immunoperoxidase was developed by incubation with diaminobenzidine.
Diaminobenzidine is used as the final chromogen, and hematoxylin as the nuclear counterstain.
Sections were stained with freshly prepared solution of diaminobenzidine and poststained with hematoxilin.
Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color.
This signal can optionally be amplified and visualized using diaminobenzidine precipitation of the antigen.
Diaminobenzidine ( DAB ) was used as a chromogen followed by nickel chloride enhancement for microtiter plates.
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A streptavidin horseradish peroxidase system using the substrate diaminobenzidine was used to stain the slides.
Color is developed using diaminobenzidine ( DAB ) and slides are coverslipped for permanence.
When the enzyme is a peroxidase, a preferred substrate is diaminobenzidine.
The biotin-avidin-horseradish peroxidase method with diaminobenzidine substrate was employed for antigen detection.
Stains are developed with avidin-horseradish peroxidase and diaminobenzidine.
The staining was revealed with diaminobenzidine ( Vector Laboratories ) and counterstaining was done with haematoxyline.
After washing, the membranes were revealed by the diaminobenzidine method.
Diaminobenzidine colorimetric reagent solution ( Dako ) was used.
In preferred embodiments, the use of diaminobenzidine is indicated.
Diaminobenzidine tetrahydrochloride ( DAB ) was used as chromogen.
Reaction was detected using avidin-biotin and diaminobenzidine system.
Diaminobenzidine ( Sigma ) was used as substrate, resulting in brown staining.
For example, horseradish peroxidase with hydrogen peroxide and diaminobenzidine.
The color reaction was then performed using diaminobenzidine ( DAB ) as recommended by the supplier.
Diaminobenzidine ( DAB ) was used as a chromogen.
Visualization of all three strips was facilitated by the addition of diaminobenzidine for 5 min.
Then diaminobenzidine ( DAB ) staining was permitted for maximum 15 minutes at room temperature.
The immunoperoxidase reaction colour was developed by incubating the slides with diaminobenzidine for 7 min.
Diaminobenzidine ( DAB ) was used as the chromogen and the sections were counterstained with 1 % methylgreen.
For immunohistochemistry, the avidin-biotin complex method was used with peroxidase and diaminobenzidine as the chromogen.
The sections were then exposed to diaminobenzidine ( Pierce ) for 5 minutes and then washed in PBS.
The binding of antibodies were visualized with diaminobenzidine ( Pierce ).
Nickel-intensified diaminobenzidine ( DAB ) was used as the chromagen to yield a gray-black precipitation product.
Immunoreactivity was revealed by the ABC method using diaminobenzidine ( brown precipitate ) as the chromogen.
A horseradish peroxidase-conjugated secondary antibody was added, followed by color development with diaminobenzidine ( DAB ).
Color reaction was developed with 3,4 diaminobenzidine in a standard procedure.
Visualization of the bound anti-HSV-HRP conjugate was facilitated by the addition of diaminobenzidine ( DAB ).
TUNEL positive cells are detected with diaminobenzidine and H2O2 according to the supplier 's instructions.
Staining was visualized with an avidin-biotin-peroxidase system ( Vector Laboratories ) with diaminobenzidine as the chromogen.
Diaminobenzidine ( DAKO, Carpenteria, CA ) is used as chromogen and hematoxilin as counterstain.
Thaw a frozen aliquot of diaminobenzidine ( DAB ).
Diaminobenzidine tetrahydrochloride ( DAB ) / hydrogen peroxide was used as colour-development HRP substrate.
Revelation of the enzyme activity by diaminobenzidine ( DAB ) . Results.
Diaminobenzidine was used as the chromogen in a commercial metal enhanced system ( Pierce Chemical, Rockford, IL ).
Blots were developed with diaminobenzidine ( DAB ).
