Examples of 'digoxigenin' in a sentence
Meaning of "digoxigenin"
digoxigenin (noun) - a steroid found in the foxglove plant, commonly used in molecular biology techniques as a detection reagent for nucleic acid hybridization
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- A steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata.
How to use "digoxigenin" in a sentence
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digoxigenin
Labeling with digoxigenin was confirmed using a hybridization filter.
The probe is labelled with either biotin or digoxigenin.
Digoxigenin for example can be recognized by antibodies against digoxigenin.
Preferably the aglycone derivative is digoxigenin.
Digoxigenin was the binary label.
In one embodiment the hapten is biotin or digoxigenin or theophylline or carborane.
Alternatively a digoxigenin label can be used which can be detected by spectrophotometery.
Ouabain is washed out at a rate between that of digoxin and digoxigenin.
In one embodiment the hapten is biotin or digoxigenin or theophylline or fluorescein or bromodeoxyuridine.
Preferred detectable labels of the invention include biotin and digoxigenin.
Polyclonal antibodies specific for digoxigenin are widely used in diagnostic assays.
This process was repeated for the next two spots above digoxigenin.
Monoclonal antibodies are labeled with digoxigenin according to the same procedure.
The digoxigenin binding modules can be connected to cell targeting entities in a variety of formats.
Monoclonal antibodies have been labeled with digoxigenin according to the same procedure.
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The conjugation with digoxigenin does not affect the activity of most therapeutic or diagnostic agents.
This precipitation removes unconjugated excess digoxigenin from the labelled probe.
Digoxigenin is detected using labeled anti-digoxigenin antibodies.
Another type of indirectly labeled oligonucleotides may include those conjugated to digoxigenin or dinitrophenyl.
DNA labeled with digoxigenin and biotin.
In a further embodiment said antibody comprises humanized IgG with two digoxigenin binding sites.
The antigenic digoxigenin moiety was recognized by the antibody-enzyme conjugate.
Briefly the assay uses oligonucleotide hybridization probes conjugated to Digoxigenin.
Whole fixed embryos were hybridized with digoxigenin containing RNA probes.
Illustrative nonradioactive moieties include biotin, fluorescein, and digoxigenin.
Furthermore, a system such as digoxigenin could be employed.
The antigenic digoxigenin moiety is recognized by the antibody-enzyme conjugate.
Functionalization with Digoxigenin.
By contrast, aglycones such as digoxigenin and digitoxgenin showed no isoform selectivity.
In another embodiment, the ligand may be fluorescein or digoxigenin or an antigen.
Similarly, probes containing digoxigenin may be detected by using a labeled antidigoxigenin antibody.
In another preferred embodiment, the moiety is digoxigenin or six histidines.
Biotin or digoxigenin end-labeled nucleic acids can be prepared according to standard techniques.
Non-radioactive labels such as digoxigenin may also be used.
In one example said fluorophore is a hapten, for instance biotin or digoxigenin.
In one embodiment of this example, digoxigenin is attached to the nucleic acid probe.
As described herein, the first analyte 's first interaction unit is a digoxigenin label.
Monoclonal antibodies are labeled with digoxigenin according to the same procedure . Example 3.
Examples of such indirect detectable groups are for example haptens, like digoxigenin or biotin.
For example a biotin, digoxigenin or digoxin labeled dNTP could be added to PCR reaction composition.
A particularly common hapten-antihapten system uses biotin and digoxigenin as reporter haptens.
The 3 B-maleiimide activated digoxigenin product eluted as a pale yellow band after two impurity bands.
The probe can carrying a number of detection moieties including radioisotopes, biotin and digoxigenin.
Oligonucleotides labelled at the 5 ' terminus with Biotin or Digoxigenin were purchased from Eurogentec.
The method of claim 7, wherein the detectable label comprises one of a peroxidase conjugate and digoxigenin.
In this case, digoxigenin immune-reacts with an alkaline phosphatase-labeled anti-digoxigenin antibody.
For example, one probe strand can be biotin labeled and the other digoxigenin labeled.
The probe was labelled with digoxigenin ( dig, Boehringer Mannheim ) following the manufacturer 's specifications.
Examples of non-radioactive isotopes for labeling a marker polynucleotide probe include biotin and digoxigenin.
The DNA was then degraded with Dnase-1, and unincorporated digoxigenin was removed by ultrafiltration.
