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diii
Machinery operator for panel processing diii.
Treatment with Hin dIII failed to cleave this plasmid.
Fused in the proper orientation and flanked by Hin dIII sites.
The Hin dIII site was filled in with Klenow reagent.
The end of the synthetic fragment terminated in a Hin dIII restriction site.
Hin dIII and Bam HI restriction sites are also indicated.
The first primer incorporates a Hin dIII site into the fragment.
All the DNAs used in the blots shown here were digested with Hin dIII.
A unique Hin dIII restriction site is present between the CpTi and NPT II genes.
Fusions with long fibres also contain this Hin dIII site.
Chromosomal DNA was digested with Hin dIII and hybridized with the indicated probe.
This was followed by a double digest of Pst i and Hin dIII.
Hin dIII cleaves the phage in the polylinker region 5ʹ of the initiation codon.
The ubiquitin cDNA clone did not contain Eco RI and Hin dIII sites.
The appropriate insertion yields a Hin dIII fragment of approximately 7 kb in size.
See also
Insert size and orientation was confirmed by digestion with Eco RI and Hin dIII.
A lambda / Hin dIII molecular weight marker was used for comparison.
The sequences commence at an Eco RI restriction site and end with a Hin dIII site.
The isolated cDNA was then inserted into a Hin dIII site of the retrovirus vector pLS1ARNL.
The plasmid was then partially digested with Pvu II and completely digested with Hin dIII.
Lane D contains pAEGIpyr-3 digested with Hin dIII to provide appropriate size markers.
The DNA was further digested with Hin dIII.
The Hin dIII site of pRKDBam/Sma3 was converted to a Hpa I site using a converter.
These fragments were removed by restriction endonuclease digestion with Hin dIII and Xba I.
Hin dIII restriction enzyme fragments of pNAPT-2 are ligated into pPNEO / ori.
The PCR product was subsequently cloned using the restriction endo-nuclease sites XbaI and Hin dIII.
Size markers ( kb ) are Hin dIII restriction fragments of Lambda-DNA.
The segment begins with the XbaI-restriction site and ends with the Hin dIII restriction site.
The plasmid pUC19 was digested with Hin dIII and the linear vector fragment was gel-purified.
Both cre genes are contained in an Eco RI - Hin dIII fragment.
A Hin dIII site exists at the C-terminus of the HIV-1 envelope gene.
Plasmid pYI016 was partially digested with Hin dIII and subsequently digested with Eco RI.
Mini-prep DNA was isolated from these six clones, digested with Hin dIII.
The forward primer added a Hin dIII site and an E. coli consensus RBS to the 5 ' end.
Colonies were blotted onto nylon filters and probed with a 1.2Kb Hin dIII VIP3A ( a ) derived fragment.
This is accomplished by Hin dIII digestion of plasmid pOW1065 prior to transformation.
The sequence of primer 88C-rlb contains an internal Hin dIII cloning site ( underlined ).
A Bam III / Hin dIII fragment of 4.5 kb was selected for cloning.
M, DNA sizing markers ( Hin dIII digest of λDNA ).
The ptg155PRO Hin dIII site is 86 bp downstream of the rabies G translation initiation codon.
The active gene is located on a 2.4 Kb basepair Hin dIII fragment.
Chromosomal DNA was digested with Hin dIII and Sma I and fractionated on a 0.5 % agarose gel.
The Hin dIII termini of pSCA111 were blunted using a DNA blunting kit ( Takara Shuzo ).
This DNA fragment was inserted at the Hin dIII site of pUC100.
This pTOK150 was digested with Hin dIII and treated with T4 DNA polymerase.
The 750 bp fragment was subcloned into a Bluescript-derived vector as a Sal I / Hin dIII fragment.
The product DNAs were digested with Hin dIII and Bam HI and cloned into M13mp19.
The isolated fragment was cloned into plasmid pTma12-1 digested with Nco I and Hin dIII.
Strain EG4961 was extracted and digested with Hin dIII plus Eco RI restriction enzymes.
