Examples of 'directed mutagenesis' in a sentence
Meaning of "directed mutagenesis"
Directed mutagenesis is a technique used in genetics and molecular biology to introduce specific changes or mutations in the DNA of an organism. It involves the targeted alteration of genetic material with the goal of studying the effects of these changes on gene function or protein structure
How to use "directed mutagenesis" in a sentence
Basic
Advanced
directed mutagenesis
This would be achieved by site directed mutagenesis.
Site directed mutagenesis was performed on the arch region.
The mutants were obtained by site directed mutagenesis.
Site directed mutagenesis.
Construction of vector for site directed mutagenesis.
By site directed mutagenesis the two amino acids were substituted separately.
Replacements were made by directed mutagenesis techniques.
This will facilitate manipulation of cellular lifespan through site directed mutagenesis.
Here random and directed mutagenesis was used to create improved variants of DsRed.
This is accomplished by conventional methods of site directed mutagenesis.
In vitro site directed mutagenesis allows to exchange desired nucleotides within a plasmid backbone.
The alterations may be induced by random or directed mutagenesis.
Subsequent site directed mutagenesis may be used to alter additional nucleotides in any mutant.
Such alteration may be accomplished by site directed mutagenesis.
Site directed mutagenesis in these helical regions could block the ability to dimerize.
See also
The detoxified form can be obtained by directed mutagenesis.
Site directed mutagenesis on these sites inactive the enzyme both in vivo and in vitro.
Of particular interest is oligonucleotide directed mutagenesis.
Site directed mutagenesis of nucleic acids and subsequent expression of the proteins is another route.
These hypotheses could then be verified with directed mutagenesis.
Describes the use of site directed mutagenesis for the purpose of reducing IgE binding.
The nucleotide sequence can be modified by site directed mutagenesis.
Site directed mutagenesis may then be achieved using specific mismatched synthetic oligonucleotides by standard methods.
This strain is used for plasmid preparation and directed mutagenesis.
Mutations were made using standard site directed mutagenesis techniques with the appropriate mutagenic primers.
Galactose positive mutants can also be generated by site directed mutagenesis.
Secondly it may be achieved by site directed mutagenesis of the same coding region.
Single stranded template was prepared and the mutation made by oligonucleotide directed mutagenesis.
Directed mutagenesis techniques have been reviewed by Lather et al.
Such plant can also be obtained by in situ site directed mutagenesis.
Site directed mutagenesis was as described in Kramer et al ref.
It consists of an effective alternative strategy to directed mutagenesis and rational protein design.
Other site directed mutagenesis techniques can also be employed with CKI nucleotide sequences.
The generation of new DsRed variants is likely to involve both random and directed mutagenesis.
This can be done by site directed mutagenesis or PCR.
Site directed mutagenesis was performed by PCR using primers containing the desired nucleotide changes.
The reagents used for oligonucleotide directed mutagenesis were purchased from Amersham.
The DNA may conveniently be modified using the technique of directed mutagenesis.
The process of site directed mutagenesis follows the instruction of the kit from Strategene Corp.
The modification was introduced at the DNA level using site directed mutagenesis.
Site directed mutagenesis Site directed mutagenesis was performed by PCR.
Modifications to the DNA sequences can be made by using site directed mutagenesis.
Oligonucleotide site directed mutagenesis was performed using the mutagenesis kit supplied by Amersham International PLC.
Point mutants Most mutants were generated by means of the Stratagene QuickChange directed mutagenesis protocol.
Alternatively, site directed mutagenesis techniques may be used.
Methods of altering the DNA sequences include but are not limited to site directed mutagenesis.
Techniques in site directed mutagenesis are well-known in the art.
Appropriate DNA inserts can also be obtained which code for polypeptide having site directed mutagenesis.
Specifically, site directed mutagenesis is used to graft the CDRs onto the framework.
The three mutations were grouped on the same cDNA by mean of Oligonucleotide Directed Mutagenesis.
You'll also be interested in:
Examples of using Directed
Show more
All feedback should be directed by mail or in person to
He directed a film called day for night
Take the pills as directed by your provider
Examples of using Mutagenesis
Show more
Mutagenesis kit according to the supplierʼs instructions
Other forms of chemical mutagenesis are also available
Mutagenesis may be performed by any suitable method
