Examples of 'dithiothreitol' in a sentence
Meaning of "dithiothreitol"
Dithiothreitol: a chemical compound commonly used in biochemistry and molecular biology
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- A small-molecule redox reagent with the molecular formula C₄H₁₀O₂S₂.
How to use "dithiothreitol" in a sentence
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dithiothreitol
Dithiothreitol is added prior to the addition of protein.
The enzyme activity was greatly enhanced by dithiothreitol.
Dithiothreitol inhibited the reaction.
The symmetric bisconjugate was removed by dithiothreitol treatment.
Dithiothreitol may be used in particular.
This inhibition was partly counteracted by dithiothreitol.
An antioxidant such as dithiothreitol may be present to prevent protein oxidation.
Deterioration of the compounds could be prevented by low concentrations of dithiothreitol.
The function of the dithiothreitol is to protect sulfhydryl groups in a protein.
In a further preferred embodiment said thiol containing compound is cysteine or dithiothreitol.
The thiols are then reduced with dithiothreitol and labelled by monobromobimane.
Dithiothreitol showed the strongest mucolytic and viscosity reducing activity.
The function of ethylene glycol is to prevent dithiothreitol from oxidation by oxidizing agents.
Cysteine and dithiothreitol did not have any effect on the enzyme activity.
Modification does not cause dissociation of the enzyme and is reversible with dithiothreitol.
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A method using dithiothreitol to reduce disulfide bonds in the antibody fragment.
Ethylene glycol should be present in an amount sufficient to protect dithiothreitol from oxidation.
Fifty µl of freshly prepared dithiothreitol was added to each cuvette with gentle mixing.
Activation of these enzymes by reduced ferredoxin and thiol s such as dithiothreitol has been reported.
Based on the effect of the dithiothreitol calcium and dimethylsulfoxide on the cellular walls.
No such disulfide cleavage occurs in the case of indoleacryloyl chymotrypsin upon treatment with dithiothreitol.
Reducing agents such as cysteine and dithiothreitol did not enhance the enzyme activity.
A disulfide bond between the biotin and the antigen molecule allows mild elution with dithiothreitol.
Dithiothreitol was purchased from Fluka.
Inclusion bodies were resuspended in guanidine hydrochloride containing dithiothreitol and solubilized at room temperature.
Dithiothreitol caused some inactivation of NAGase 2 while stimulating activity of NAGase 1.
Compounds of the general formula E are then prepared by reduction of D with dithiothreitol in methanol.
The final concentration of dithiothreitol was then adjusted to 1 mM.
In one embodiment, the reducing agent is dithiothreitol.
Nucleotides, radionucleotides, and dithiothreitol were added immediately prior to use.
The buffer may be a reducing reagent, such as dithiothreitol.
Preferably, this complex also comprises dithiothreitol in a concentration of the order of 1 mM.
A process according to claim 1 in which the reducing agent is dithiothreitol.
Reduction of the disulfide group with dithiothreitol gave the thiol-containing maytansinoids.
The buffer may contain a reducing reagent, such as dithiothreitol.
One millimolar dithiothreitol is included in the second buffer change only, for a total of 3 hours.
Preferably, mercaptoethanol and dithiothreitol are used.
Propyl gallate optionally in the presence of butylated hydroxyanisole or butylated hydroxytoluene ; and dithiothreitol.
The disulfide can be cleaved with dithiothreitol or other suitable agent, exposing a reactive thiol group.
The enzyme is inhibited by 1 mM dithiothreitol.
Unreacted cobalt was detected with dithiothreitol which forms a brown-colored product with cobalt ions.
The labelling was terminated by addition of 10 µmol of dithiothreitol per mg of protein.
Muneta also indicated that cysteine, dithiothreitol and two sulfhydryl compounds temporarily inhibited the blackening.
A method according to claim 1, wherein the reducing agent comprises dithiothreitol.
Both N-acetylcysteine and dithiothreitol restored the acetaminophen-induced protein sulfhydryl loss.
A process according to claim 4 in which the cofactor is reduced glutatione or dithiothreitol.
MAC-2 was reduced with dithiothreitol and cysteine residues were alkylated by carboxymethylation with iodoacetamide.
A process according to claim 8, wherein the sulfhydryl reducing agent comprises dithiothreitol.
Finally, dithiothreitol is added to a final concentration of 1mM.
The method of claim 1 wherein said disulfide bond reducing agent is dithiothreitol.
