Examples of 'dntps' in a sentence
Meaning of "dntps"
dNTPs is a noun that stands for deoxynucleoside triphosphates, which are the building blocks of DNA. In molecular biology, dNTPs are essential for DNA replication and amplification processes, as they provide the necessary nucleotide units for synthesizing new DNA strands
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- plural of dNTP
How to use "dntps" in a sentence
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dntps
All dNTPs are unlabelled in this type of assay.
The kit can further comprise dNTPs.
Unincorporated dNTPs are washed away between each of the nucleotide additions.
The primer was extended by the incorporation of dNTPs.
Preferably the labelled oligonucleotides or dNTPs are labelled with biotin.
Matching probes are elongated using unlabeled dNTPs.
The reaction uses dNTPs as substrates and results in a release of pyrophosphate.
Collectively these may be referred to as dNTPs.
Hydroxyurea decreases the production of dNTPs by inhibiting the enzyme ribonucleotide reductase.
The primer is not elongated in the absence of added dNTPs.
One or more of the dNTPs may be replaced with a suitable analogue thereof.
This step is most useful if primers or dNTPs have been tagged.
Nucleic acid synthesis proceeds by extension of each primer with a polymerase and the four dNTPs.
The beads were incubated with the dNTPs for one minute and removed.
Synthesis using a DNA polymerase in the presence of the four dNTPs.
See also
References to NTPs herein specifically include references to dNTPs unless context dictates otherwise.
DNTPs are added individually and the incorporation results in a light signal which is measured.
An exemplification of the use of two labelled and two unlabelled dNTPs is described below.
The dNTPs and ddNTPs used in the sequencing reactions were purchased from Boehringer.
A small amount of one ddNTP is included with the four conventional dNTPs in a polymerization reaction.
DNTPs are added individually and the incorporation results in a light signal that is measured.
An alternative labeling method incorporates a label into the dNTPs which are used for correction.
PriSL is thought to initiate primer synthesis with NTPs and then switch to dNTPs.
There is increased amount of dNTPs and SSII reverse transcriptase.
The NcoI site of this fragment was filled in with Taq polymerase and dNTPs.
Also added to the reaction mixture are dNTPs and a first DNA polymerase.
These mutants have an RT with enhanced ability to discriminate between NRTIs and native dNTPs.
Active clones provide the dNTPs necessary for Tag polymerase to amplify the ndk gene.
The FcoRI ends were filled with dNTPs and Klenow.
The Clontech reverse transcriptase and dNTPs were substituted with Superscript II and dNTPs from Invitrogen.
In vitro PCR amplification is performed under conditions offering any of the standard nucleotides dNTPs.
Telomerase requires dNTPs to synthesize DNA.
Dideoxynucleotides have a substituted 3 ' position in comparison to conventional dNTPs.
Compartments also contain Taq Pol and dNTPs and telomere specific primers.
In one aspect, the component for amplification comprises dNTPs.
Preferably, the dNTPs are supplied in a buffered solution with a reference dye.
Experiments were run in parallel using either natural or base-modified dNTPs.
Synthesis of the esters of dNTPs and caproic acid, amidated by the fluorophores,.
The kit of claim 4 further including hapten labeled ddNTPs or dNTPs.
One or more of the dNTPs and / or NTPs may be substituted by a suitable analogue.
In a second approach, incomplete or fractional substitution of respective dNTPs can be applied.
The primers, detectable oligonucleotides and dNTPs can be packaged in various configurations.
After you lay down a primer, you can add dNTPs.
The method of 1 wherein fluorescently labeled dNTPs or dNTPs are incorporated into the amplicons.
Influences of the hybridization temperature and of the concentration of dNTPs and MgCl2.
According to the present method, dNTPs used in the extension step are preferably labeled.
The method of claim 8 wherein the biotin is associated with dNTPs which are incorporated.
The dNTPs have a 3 ' azide block as well as the fluorescent label attached to the base.
Preferably, the primers, detectable oligonucleotides and dNTPS are in a single container.
For instance, four types of modified dNTPs containing a terminator that blocks further polymerization are added.
