Examples of 'dsba' in a sentence
Meaning of "dsba"
Dsba (noun): Acronym that may have multiple meanings based on the context, not defined in English
How to use "dsba" in a sentence
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dsba
Present during the production of the DsbA.
Native DsbA protein was purified by anion exchange chromatography.
The chromatography process resulted in ultrapure DsbA.
Purified DsbA protein was generated.
Present during the production of the DsbA or DsbC.
Evaluation of DsbA and DsbC removal during purification of a monoclonal antibody.
The mock pool primarily contains the DsbA molecule.
The DsbA is eluted from the column with a salt gradient.
No aggregation issues were observed with the purified DsbA material.
Ultrapurified DsbA and DsbC and methods of making and using the same.
In some embodiments a preparation of polyclonal antibodies specifically binds DsbA or DsbC.
The fractions containing the DsbA protein were pooled based on size exclusion chromatography data.
Such compositions are used to generate antibodies that are highly specific to DsbA or DsbC.
DsbA is eluted from the column using a step gradient increase in salt concentration.
Provided herein are methods for generating ultrapure preparations of DsbA and DsbC.
See also
DsbA is a strong thiol oxidant and the intermediate donor of disulfide bonds to secreted proteins.
Most preferred is a phage or phagemid vector comprising the signal sequence DsbA or a homolog thereof.
In some embodiments, DsbA and DsbC are derived from bacteria.
Loading buffer is the buffer used to load the composition comprising DsbA onto a chromatography material.
Polypeptides for which DsbA and / or DsbC may be used in production.
In some embodiments, the bacterial cell overexpresses DsbA.
In some embodiments, the DsbA is expressed in cell.
In some embodiments, the invention provides ultrapure preparations of DsbA.
In contrast, the signal sequence from DsbA promotes efficient translocation of TrxA.
In some embodiments, the detection antibody specifically binds ultrapure DsbA.
In some embodiments, the detection antibody that binds DsbA is conjugated to a horseradish peroxidase.
In some embodiments, the capture antibody specifically binds ultrapure DsbA.
A purification scheme for histidine-tagged DsbA using immobilized metal affinity chromatography is provided.
The process of claim 1 wherein nucleic acid encoding DsbA is expressed.
In some embodiments, the DsbA is eluted from the cation chromatography material using a salt gradient.
In some embodiments, the será comprises polyclonal antibodies that specifically bind DsbA.
Contacting a detection antibody that binds DsbA with the sample-capture antibody combination material ;.
In some aspects, the invention provides methods for generating ultrapure preparations of DsbA.
The DsbA is eluted from the anion exchange chromatography material with the following steps,.
In some embodiments, the antibodies that specifically bind ultrapure DsbA are polyclonal antibodies.
Anti-DsbA antibody was produced by immunizing rabbits with purified DsbA.
In other embodiments, the antibodies that specifically bind ultrapure DsbA are monoclonal antibodies.
In some embodiments, the DsbA and DsbC polypeptides are E. coli DsbA and DsbC polypeptides.
Such proteins that enhance refolding include, for example, DsbA or DsbC proteins.
In some embodiments, monomeric DsbA or monomeric DsbC is determined by SEC.
The method of claim 2, wherein the recombinant polypeptide is prepared in a host cell overexpressing DsbA.
Figure 4 shows a western blot of DsbA Poros HS pool fractions.
The DsbA was extracted from E. coli cell paste using homogenization and centrifugation.
In an embodiment, the chaperone protein is DsbA and / or DsbC.
In some embodiments, DsbA and C are expressed in the bacterial host cell.
In some embodiments, the bacterial cell overexpresses DsbA and / or DsbC.
An exemplary DsbA protein is E. coli DsbA.
Three rabbits ( per immunogen ) were immunized with either DsbA or DsbC.
Total relative DsbA content ( ng DsbA / mg total protein ) of purification fractions.
In Gram-negative bacteria, the synthesis of disulfide bonds is catalyzed by the thiol-disulfide oxidoreductase DsbA.
