Examples of 'elispot' in a sentence
Meaning of "elispot"
Elispot: A noun that typically refers to a laboratory technique used to detect and quantify the secretion of proteins, such as cytokines, from individual cells
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- enzyme-linked immunospot assay
How to use "elispot" in a sentence
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elispot
O ELISpot assay was performed on pooled splenocytes of mice.
The FluoroSpot assay is very similar to the ELISpot assay.
The ELISpot assay was conducted as follows.
The spots were visualized and counted using an ELISpot reader.
The EliSpot for.
Strong and broad cellular immune responses measured by ELISpot.
The ELISpot assay was repeated three times in three separate experiments.
Cells are plated out and the number infected is determined by ELISpot.
LTT and EliSpot are a few of these tests.
Splenocytes were collected after the final treatment and cellular responses were analyzed by ELISpot.
The Elispot assay is widely described in the documents of the prior art.
Cellular responses were examined using Elispot assay.
The Elispot is an even more reliable test but is not done in all countries.
HA is no exception as no SFCs were detected in our ELISpot assay.
ELISpot assays were performed to determine cellular mediated immune responses in vaccinated subjects.
See also
The mixture was then transferred into ELISpot plates preadsorbed with Tat.
The induction of the cellular immune response was evaluated after each immunization by IFNY ELISpot.
Spleen cell cultures for cytokine analysis by Elispot Mice were killed by cervical dislocation.
The immune response induced was measured one week following immunization using IFN ELISpot assays.
Elispot method is known as an alternative to a test for cytotoxic activity J.
I recommend that funding go toward evaluating the ELISpot test in our Canadian population.
By ELISpot analysis Fig.
The cells may then be assessed for infection by ELISpot or Western blot as appropriate.
After incubation, the ELISpot plates were developed according to manufacturer 's instructions.
Patient PBMC were taken from liquid nitrogen and thawed the day before the ELISpot assay.
Read on an AIDS ELISpot reader.
Ag-specific responses were quantified by ELISpot.
Elispot assays were performed in duplicate on splenocytes 10 days after the last immunisation.
Isolated PBMC are then used immediately for an Elispot assay.
Having observed substantial ELISpot responses, we next examined additional parameters of cellular immunity.
IFNY production was measured by ELISpot.
In a preferred embodiment, ELISpot is used to assess the cytokine expression and / or chemokine expression.
TCR avidity was assessed by IFN ELIspot.
Representative ELISpot results are shown in Figure 2.
The spots were counted using a Carl Zeiss Visión Elispot reader unit.
IFN-g was measured by ELISpot using commercially available kits.
The spots are visualized and counted using an ELISpot reader ;.
The Elispot assay was performed according to the manufacturer 's recommendations.
Cellular immune responses are detected using Interferon-Y ELIspot assays.
IFNY levels measured after 18 hours by ELISpot and expressed as spots per million splenocytes.
The production of interferon-gamma by these primate cells was measured using ELIspot assays.
T cell activation was determined by ELISpot measuring secreted IFN-Y.
Following a suitable length of time, the extent of infection is determined using the ELISpot method.
IFNY ELISpot was used to evaluate antigen-specific cellular responses 1 week after the last immunization.
Antigen-binding cell lines were confirmed by ELISpot assay and tetramer staining.
Importantly, we also observed discrepancies between the outcomes obtained by skin test and ELIspot.
IFN-Y secreting cells were determined by ELISpot assay as described above.
In a preferred embodiment, step c of detecting T cell responses is performed by ELISpot.
Most importantly however, interferon-gamma ELIspot responders were observed exclusively in the verum groups Fig.
The production of interferon-gamma by these porcine cells has been measured using ELIspot assays.
