Examples of 'elution buffer' in a sentence
Meaning of "elution buffer"
Elution buffer is a solution used in various scientific techniques, such as chromatography, to separate components of a mixture. It is designed to disrupt the interactions between the target molecule and the stationary phase, allowing the target molecule to be eluted or released. The elution buffer typically contains specific salts, solvents, or pH-adjusting agents that promote the elution process
How to use "elution buffer" in a sentence
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Advanced
elution buffer
Any suitable elution buffer can be employed.
The mRNA precipitate was dissolved in an elution buffer.
The elution buffer has a basic pH.
The immunoglobulin with an appropriate elution buffer.
An elution buffer is then added.
In some embodiments the elution buffer is water.
The elution buffer may have an increased pH or salt concentration.
The protein then is dissolved in elution buffer.
The elution buffer is applied to the matrix without disturbing it.
Adherent phage are eluted using elution buffer.
The elution buffer can be selected using routine experimentation.
Then an elution step is effected by injecting elution buffer.
The pH value of such an elution buffer is preferably alkaline or neutral.
It was not necessary to include a buffer in the elution buffer.
In a further embodiment the elution buffer is a sodium phosphate buffer.
See also
The protein was eluted with the elution buffer.
An elution buffer may comprise one or more additional components.
Hp was then eluted with elution buffer.
The first elution buffer may have different components.
This is normally achieved using a suitable elution buffer.
After the elution buffer was applied the column is centrifuged twice.
The column is eluted using an appropriate elution buffer.
An elution buffer not containing salt may also conveniently be used.
This solution was also used as the elution buffer.
The pH value of such an elution buffer is in some embodiments alkaline or neutral.
Antibody was eluted with elution buffer.
The amount of elution buffer used is desirably kept as low as possible.
The elution procedure was repeated with fresh elution buffer.
The use of elution buffer without NaCl was carried forward as well.
Antibody elution is achieved by adding elution buffer and incubating.
The elution buffer is added and mixed by the automated robotic device.
Daptomycin is eluted from the column with elution buffer.
The ionic strength of the elution buffer may be provided by various salts.
Then the column is flushed with the elution buffer.
The elution buffer was added and mixed by the automated robotic device.
The product is then eluted with the elution buffer.
An elution buffer.
Samples of the tested variants were pure and supplied in elution buffer.
Often the loading buffer and elution buffer will be the same.
Compounds which remain bound can be released by a suitable elution buffer.
The pump was reversed and the elution buffer recovered for analysis.
The preferred fractions can also be pooled in the elution buffer.
The use of triethanolamine as an elution buffer allows direct lyophilization of the eluted product.
Other antibody isotypes were collected by switching to the IgG elution buffer.
To improve the elution step the elution buffer may comprise salt.
In another embodiment there is provided a purification intermediate comprised in an elution buffer.
The nucleic acid is eluted with an elution buffer and recovered.
Therefore in one embodiment there is provided a clostridial neurotoxin comprised in an elution buffer.
The elution buffer with the lysate is then dispensed in the analysis tube.
Eluted from the second solid support in a basic elution buffer.
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Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
Examples of using Elution
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Column elution method with recirculating pump
Results obtained after elution and neutralization
Column elution method with levelling vessel
