Examples of 'endonuclease sites' in a sentence
Meaning of "endonuclease sites"
endonuclease sites: Specific locations where endonucleases (enzymes that cleave DNA or RNA) bind and cleave nucleic acids
How to use "endonuclease sites" in a sentence
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endonuclease sites
Relevant restriction endonuclease sites are also shown.
Thin vertical lines indicate constant restriction endonuclease sites.
Selected restriction endonuclease sites arc marked.
Endonuclease sites used in the invention were chosen according to a hierarchy of occurrence.
Important restriction endonuclease sites are indicated.
Restriction endonuclease sites and Kozak consensus region which were supplied by synthetic oligonucleotides are indicated.
Selected restriction endonuclease sites are marked.
Polymerase chain reaction will be performed to amplify the DNA with certain restriction endonuclease sites.
The restriction endonuclease sites were confirmed by digestion.
For instance they may introduce or remove restriction endonuclease sites or alter codon usage.
Restriction endonuclease sites can be incorporated into the primers.
These oligonucleotides contain unique restriction endonuclease sites needed for the assembly.
Restriction endonuclease sites are located on the DNA as shown.
The locations of 6 basepair restriction endonuclease sites are noted.
The restriction endonuclease sites used for cloning are boxed.
See also
Alternatively, the primers optionally incorporate restriction endonuclease sites.
The predetermined restriction endonuclease sites in the modules are unique within the module.
Preferably, said polynucleotide sequence to be linearized is flanked by two endonuclease sites.
Additional restriction endonuclease sites were also incorporated into PCR primers to facilitate subsequent cloning.
The piH3M vector of the present invention is preferred, and employs the inverted endonuclease sites.
The restriction endonuclease sites needed for cloning are introduced vÃa the following PCR primers.
Figure 15A is a physical map showing restriction endonuclease sites of plasmid pSuFel.
Restriction endonuclease sites flanking the Bst XI cloning sites are shown.
Conventionally, this could be done by incorporation of restriction endonuclease sites into the DNA template.
No Ear I restriction endonuclease sites such that insertion of sequences would produce a unique site.
In this way, plasmid pNPI121 with these restriction endonuclease sites eliminated was obtained.
Restriction endonuclease sites are also shown, for example, in Figure 2.
Figure 15D is a physical map showing restriction endonuclease sites of plasmid pSuFe4.
Restriction endonuclease sites used for Maxam-Gilbert DNA sequence analysis of the 1.1 kb cDNA are shown.
Other methods have employed restriction endonuclease sites to separate a donor / acceptor pair.
Vertical arrows indicate polymorphic ( C / T ) sequences giving rise to polymorphic restriction endonuclease sites.
The primers may also carry restriction endonuclease sites for use in cloning the amplified DNA.
Figure 10A is a physical map showing restriction endonuclease sites of pSU17.
There must be unique restriction endonuclease sites on each side of the identified mutation site ( s ).
Each 5 ' and 3 ' PCR primer contains artificial restriction endonuclease sites for cloning purposes.
Abbreviations for restriction endonuclease sites in the drawings are as follows,.
Figure 2 shows examples of restriction endonuclease sites for Hpa I ;.
The Nco I and Sfi I restriction endonuclease sites at 5 ' and 3 ' were included in the synthesized fragments.
Figure 10F is a physical map showing restriction endonuclease sites of pCab-SU13.
Finally, all but one remaining restriction endonuclease sites were removed in every gene sequence.
Figure 16B is a physical map showing restriction endonuclease sites of plasmid pCS325.
Fig . 2 shows the restriction endonuclease sites of the pag C locus.
Figure 16A is a physical map showing restriction endonuclease sites of plasmid pPAT108.
For example, appropriate restriction endonuclease sites can be engineered ( e.g., using PCR ).
Figure 10B is a physical map showing restriction endonuclease sites of pSSU-SU11.
For example, by using the indicated restriction endonuclease sites flanking the promoter, R.B.S. e . g.
Figure 25A is a physical map showing restriction endonuclease sites of plasmid pZ6A-SU1.
Figure 25B is a physical map showing restriction endonuclease Sites of plasmid pZ6AT-SU1.
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Examples of using Endonuclease
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Homing endonuclease recognition sites are extremely rare
Preferably the enzyme is a homing endonuclease or a meganuclease
The endonuclease enzyme restriction sites are underlined
