Examples of 'exchange chromatography step' in a sentence
Meaning of "exchange chromatography step"
Exchange chromatography step: A chromatography technique that separates molecules based on their interactions with a stationary phase during the exchange step
How to use "exchange chromatography step" in a sentence
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exchange chromatography step
Preferably said capture step is a cationic exchange chromatography step.
The final cation exchange chromatography step after refolding was also omitted.
Subsequent purification to homogeneity was carried out using an ion exchange chromatography step.
The ion exchange chromatography step was repeated if the desired purity was not achieved.
This step may be prior to or after the anion exchange chromatography step.
A suitable anion exchange chromatography step would be the same as those discussed herein.
Purity can be further increased by means of a second cation exchange chromatography step.
The ion exchange chromatography step may contain an anion exchanger or a cation exchanger.
This step can be performed before or after the anion exchange chromatography step.
This anion exchange chromatography step may also follow the treatment with a DNase solution.
Process changes in this example are predominantly in the preparative anion exchange chromatography step.
The cation exchange chromatography step may be performed using any suitable cation exchange matrix.
Depolymerisation from polysaccharide to oligosaccharide can occur before or after the anionic exchange chromatography step.
A weak anion exchange chromatography step was followed to reduce HMW species.
Recovering the purified antibody from the effluent of the charged ion exchange chromatography step.
See also
For example, an anion exchange chromatography step may be present.
An exclusion chromatography step is preferentially carried out immediately after the anion exchange chromatography step.
In the latter case, the anion exchange chromatography step is preferably included.
In certain embodiments, it is preferred to use at least one anion exchange chromatography step.
The cation exchange chromatography step removes protein and non-protein impurities.
Preferably, the capture step is a cationic exchange chromatography step.
Preferably, the cation exchange chromatography step is followed by the anion exchange chromatography step.
According to the invention, a following step can be an anion exchange chromatography step.
However, typically the anionic exchange chromatography step will be carried out once.
For adenovirus purification, it is preferred to use at least one anion exchange chromatography step.
The cation exchange chromatography step may follow the anion exchange chromatography step, or vice versa.
Selected fractions are pooled and forwarded to a cation exchange chromatography step on a sulphonate column e . g.
An anion exchange chromatography step on Q Sepharose was performed as described in Example1.
One such step includes, but is not necessarily limited to, a cation exchange chromatography step.
In a first procedure, the anion exchange chromatography step is carried out downstream from the TFF.
Obviously, other purification steps may also be suitably combined with an anion exchange chromatography step.
The yield of the anionic exchange chromatography step is typically greater than 70 % e . g.
Process changes as compared to Example 5 were predominantly in the preparative anion exchange chromatography step.
The ion exchange chromatography step is preferably carried out using a carboxymethyl ( CM ) - resin.
To separate ANP from these substances, a second cation exchange chromatography step may be used.
This cationic exchange chromatography step was performed at 2-8°C.
In a particularly relevant embodiment, an anion exchange chromatography step precedes step ( a ).
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