Examples of 'expression cloning' in a sentence
Meaning of "expression cloning"
Expression cloning: A molecular biology technique used to isolate and amplify specific genes or gene sequences based on their expression in a particular cell type or under specific conditions. This method allows researchers to study the function and regulation of individual genes
How to use "expression cloning" in a sentence
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expression cloning
Longer polypeptides are conveniently obtained by expression cloning.
The expression cloning was performed using standard methods.
Sublibraries were obtained and subjected to a novel round of expression cloning.
Expression cloning techniques also may be applied on cDNA libraries.
Construction of receptor expression cloning cassette and shuttle vector.
The gene encoding the receptors can be identified by expression cloning.
Expression cloning of new receptors used by simian and human immunodeficiency viruses.
These cells are also useful for expression cloning of ion channels.
Accordingly an expression cloning strategy was devised to isolate this second receptor component.
Third technique for detecting a function expressed by a microorganism consists of using expression cloning.
Usually the ultimate aim of expression cloning is to produce large quantities of specific proteins.
These monoclonal antibodies are then used in affinity purification or expression cloning of the receptor.
Expression cloning in the COS cell system is performed as follows.
This assay was utilized for expression cloning of a Nogo receptor.
Additional Chlamydia trachomatis antigens were identified by serological expression cloning.
See also
As a results an expression cloning plasmid was generated which was autonomously maintained in Aspergillus.
Example and summary of the direct expression cloning of HCAb into HEK cells.
Expression cloning was used to isolate the CAK1 cDNA.
The GHSRs were isolated and characterized using expression cloning techniques in Xenopus oocytes.
Expression cloning is available from such commercial services as Lark Technologies, Houston TX.
In another case, expression cloning methods are used.
Alternatively, antibodies may be used for expression cloning.
In another embodiment, expression cloning methods are used.
Serological identification of antigens by recombinant expression cloning ( SEREX ).
Alternatively, expression cloning methods are used.
A linear integration vector-system was used for the expression cloning of the gene.
For instance, expression cloning may be employed for this purpose.
The method of claim 1 further comprising expression cloning the antigen.
Figure 6, Expression cloning vector containing the albumin sequence illustrated in figure 2.
As an example, a radioactive slide binding cDNA expression cloning method can be used.
Figure 4, Expression cloning vector containing the Fc regions illustrated in figure 1.
NRP2 was subsequently identified by expression cloning.
This bioassay was used during expression cloning of a cDNA library generated from PU-34.
In addition, lung tumor antigens may be identified by T cell expression cloning.
The positive DNA pools obtained from the expression cloning effort described in Example 7.
Alternatively, cDNA clones encoding ACT-4 ligands can be obtained by expression cloning.
Figure 1, mouse Rdcvf1 nucleotide sequence from the expression cloning and mouse RdCVF1 amino acid sequence.
Alternatively, cDNA clones encoding ACT-4-L ligand polypeptides can be obtained by expression cloning.
In particular, the SLAM antigen has been isolated by expression cloning using a specific antibody.
The gene encoding an MCP-4 receptor can be identified by expression cloning.
This example describes preparation or a cDNA library for expression cloning of murine CD40-L.
A cDNA clone encoding this DW34 growth-stimulating factor was isolated by expression cloning.
Alternatively, LP48 receptor ( s ) can be identified using expression cloning Kitamura, Intl.
Moreover, Zcytor14 labeled with biotin or FITC can be used for expression cloning.
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