Examples of 'expression host' in a sentence
Meaning of "expression host"
expression host - In biology and biotechnology, an expression host is a cell or organism that is used to produce a specific protein or biomolecule through the genetic expression of a foreign gene
How to use "expression host" in a sentence
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expression host
Pichia pastoris has been approved as expression host.
Another suitable expression host is a bacterium.
In one embodiment the organism is an expression host.
Processing in the expression host may also be used.
A transcriptional termination region functional in said expression host.
Another suitable expression host is a fungus.
The expression host may be cultured to give a cell line.
From the expression host.
The propeptide may be homologous or heterologous to the expression host.
As expression host Aspergillus niger could be used.
A selectable marker operative in the expression host may be present.
A protein expression host has to satisfy several criteria,.
The host cell may be a cloning host or an expression host.
This cell is used as an expression host for individualising the vectorised fragments of DNA.
Sometimes it may be desirable to clone and screen in the expression host.
See also
Preferably, the expression host organism is a bacterium.
Where appropriate is related to the choice of the expression host organism.
A suitable expression host is preferably yeast, in particular a methylotrophic yeast.
Generally a selectable marker operative in the expression host will be present.
The expression host organism is understood to mean a non-human organism.
This means that it is foreign to the selected or intended expression host cell.
The expression host may be able to grow in serum-free media.
The secretion leader may be selected on the basis of the desired expression host.
In the present invention, the expression host is preferably a bacterial expression strain.
Various transcriptional initiation regions may be employed that are functional in the expression host.
In one embodiment, a homologous expression host may be used.
The expression vector bearing the synthetic gelonin gene was inserted into a suitable expression host.
If the expression host is eukaryotic, it generally is preferred that a signal sequence be encoded.
The selection of the appropriate promoter is dependent upon the proposed expression host.
In one embodiment the expression host may be able to grow in serum-free media.
In such case the vector plus insert may be transformed directly into the expression host.
Some useful expression host cells include well-known prokaryotic and eukaryotic cells.
The yeast Saccharomyces cerevisiae is another popular expression host.
The vector is introduced in a suitable expression host cell, which expresses the corresponding protease variant.
In one embodiment the xylanases taught herein are expressed in the expression host Trichoderma reesei.
Preferably the expression host cells are removed from the media before the cell disruption e . g.
Described herein, the organism is an expression host.
More preferably, the expression host organism is Escherichia coli.
A replication system functional in an expression host cell ;.
Preferably, the expression host cell comprises a Bacillus sp ., more preferably Bacillus licheniformis or Bacillus subtilis.
For example, many recombinant products can be toxic to the expression host.
A method according to Claim 1, employing as expression host a protease negative alkalophilic Bacillus strain.
Alternatively, amplification may await transfer to the secondary cell expression host.
Preferably, the expression host cell comprises a Bacillus spp ., more preferably Bacillus licheniformis or Bacillus subtilis.
Thus, the chosen signal sequence may be homologous or heterologous to the expression host.
The phages can then be used to infect an expression host such as E. coli.
In a particular embodiment, the second nucleic acid sequence is heterologous to the expression host.
In the currently preferred embodiment of this invention, expression host is bacterium Escherichia coli.
The final expression plasmids ( BcSP-Histag-xylanase ) were individually transformed into a Bacillus subtilis expression host.
A method according to Claim 1 or 2, wherein said expression host cell is a Bacillus.
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