Examples of 'expression plasmids' in a sentence
Meaning of "expression plasmids"
Expression plasmids are DNA molecules that are used in molecular biology to produce large quantities of specific proteins within cells. These plasmids are designed to contain a promoter sequence that can drive the transcription of the target gene, allowing for the production of the desired protein in a host organism
How to use "expression plasmids" in a sentence
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expression plasmids
Integrity of the expression plasmids was confirmed by sequencing.
Plasmid includes shuttle and expression plasmids.
Other yeast expression plasmids would also be suitable.
The antibodies were expressed from individual expression plasmids.
Construction of expression plasmids and mutagenesis.
Expression plasmids were prepared as described below.
Numerous suitable expression plasmids are known in the art.
Such promoters are found in commercially available expression plasmids.
The maintenance of expression plasmids was enhanced at two independent levels.
All mutations were confirmed by direct sequencing of the expression plasmids.
For yeast expression the following expression plasmids are provided as examples.
The two expression plasmids were linearized with FspI in preparation for transfection.
Main features of the expression plasmids.
Suitable expression plasmids for each of these species are known in the art.
Cloning into yeast expression plasmids.
See also
Similar expression plasmids were generated for each of the ten human frizzled proteins.
Construction of the individual subunit expression plasmids is described herein.
The expression plasmids of the invention have also been used to produce numerous fused polypeptides.
The cells were transfected separately with linearized expression plasmids.
The construction of suitable expression plasmids is well known in the art.
Coli strains suitable for protein expression were transformed with the expression plasmids.
Construction of expression plasmids.
Expression plasmids were transfected into mammalian cells which had been infected with a parent vaccinia virus.
Restriction sites used in the construction of the expression plasmids are marked.
One can thus generate expression plasmids harboring the constant regions of immunoglobulins.
One typical method for operatively linking inserts into expression plasmids is by directional ligation.
The expression plasmids and optionally other helper plasmids are transformed into a suitable prokaryotic host cell.
Preparation of reporter gene constructs and expression plasmids containing the constructs.
Plasmid rescue from the yeast transformants verified the integrity of the two expression plasmids.
We further describe a series of expression plasmids which are referred to herein as pGEN plasmids.
Plasmid rescue from the yeast transformants verified the integrity of the control and expression plasmids.
These data indicate that these two expression plasmids are able to express hLF.
Expression plasmids were transfected into cells by lipofection using DOTAP.
The construction of the other two expression plasmids Fig.
Construction of expression plasmids encoding chimeric proteins and expression in Pseudomonas.
CHO cells were transfected with the respective antibody expression plasmids.
Coli transformed with an expression plasmids encoding a soluble oxidation resistant TM analog.
Both lipases were secreted efficiently from Aspergillus oryzae transformed with lipase expression plasmids.
The expression plasmids of the invention also contain an ATG initiation codon.
The specification also describes MPSV mammalian expression plasmids containing hTRT fusión proteins.
Three different expression plasmids were utilized in the cloning of the KGF analog genes.
Human rhabdomyosarcoma cells were transiently transfected with Vpr expression plasmids and selected with puromycin.
Expression Plasmids cDNAs encoding proteins tagged with a specific epitopes were constructed in expression vectors.
Preparation of Stalkokine expression plasmids.
IRNA expression plasmids can be transfected into target cells as a complex with cationic lipid carriers e.
EXAMPLES Plasmids and DNA material All expression plasmids are of the cPOT type.
Yeast expression plasmids for Fab expression.
This resulted in 6 different expression plasmids.
Two DHFR expression plasmids were used.
Making and description of the test-polypeptide expression plasmids.
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