Examples of 'feeder layer' in a sentence
Meaning of "feeder layer"
feeder layer: A substrate or base material used in cell culture techniques to support the growth and proliferation of cells, often in research or biotechnology applications
How to use "feeder layer" in a sentence
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feeder layer
Hybridomas are typically grown on a feeder layer of macrophages.
A feeder layer of mitotically inactivated fibroblasts provides a source of cytokines.
Cultures may also be initiated using a feeder layer.
The feeder layer generally produces a broader range of phenotypes.
The hybridomas can be grown on a feeder layer of macrophages.
This feeder layer no longer appears to be necessary after a number of passages.
Step b may also comprise the withdrawal of the feeder layer.
In some cases the feeder layer may be producing growth factors or cytokines.
The dissociated cells are then grown in cultures over a feeder layer.
Thymocytes may be used as a feeder layer to condition the medium for the fused cells.
The present invention provides methods aimed at avoiding murine feeder layer impurities.
Phase contrast of the feeder layer cells is shown in Fig.
The present invention is not limited to a certain initiation stage feeder layer removal agent.
Design of a feeder layer to enhance and sustain growth of FGRS transduced ECs.
The culturing is carried out in medium containing serum on a feeder layer of irradiated fibroblasts.
See also
Similarly MEF feeder layer supplies a friendly surface for SSC attachment.
Alternative processes permit the maintenance of pluripotent cells without the use of a feeder layer.
SCM and a feeder layer.
Preferably the ES cells are grown in the presence of a fibroblast feeder layer.
In addition, after NIKS cell growth on the feeder layer, the feeder layer is removed.
HESC are plated as a single cell monolayer without the use of a stromal feeder layer.
The 3T3 feeder layer normally suppresses the growth of differentiated mesenchymal cells as human dermal fibroblasts.
Either SCM or CSCM may be used in the presence of a feeder layer.
The background MEF feeder layer remains non-fluorescent.
The subject cells may be grown in a co-culture with feeder layer cells.
Remove feeder layer cells frozen in freezing media from liquid N2 container.
After transformation, the cells are plated onto a feeder layer in an appropriate medium.
Alternatively, peritoneal macrophages or non-immune spleen cells may be used as a feeder layer.
After transfection, the cells are plated onto a feeder layer in an appropriate medium.
In a preferred embodiment, the mouse fibroblast cells form a mouse fibroblast feeder layer.
The feeder layer was prepared with agar that had been equilibrated at 42°C.
In some processes for producing definitive endoderm cells, hESCs are maintained on a feeder layer.
In the latter, the 3T3 feeder layer maintains stemness of the cells.
After 24 h the medium is replaced with fresh medium and again when used as a feeder layer.
In a more specific embodiment, said feeder layer comprises K562 cells or peripheral blood mononuclear cells.
Spleen cells from a non-immunized Balb / c mouse were also added as a feeder layer.
T cells may be used as a feeder layer at a concentration of 10 million cells/10 ml of medium.
In preferred embodiments, EDTA is used as the initiation stage feeder layer removal agent.
Feeder layer free cultures may utilize a protein coated surface, e.g. matrigel, etc.
Spleen cells from one nonimmune Balb / c mouse also were added as a feeder layer.
ADC form similar networks in the absence of a feeder layer ( Figure 7A ).
Spleen cells from one non-immune BABB / c mouse also were added as a feeder layer.
Northern Analysis Cells were grown in standard keratinocyte culture medium on a 3T3 feeder layer.
In some processes, pluripotent stem cells, e.g. hES cells, are maintained on a feeder layer.
Assays, however, were run in the absence of a 3T3 feeder layer.
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Examples of using Layer
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