Examples of 'fhbp' in a sentence
Meaning of "fhbp"
fhbp (noun) ~ Abbreviation for factor H binding protein, a bacterial protein that interacts with host factor H to evade the immune response. FHBP is often studied in the context of infectious diseases and vaccine development.
How to use "fhbp" in a sentence
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fhbp
This latter approach was undertaken with fHBP.
Chimeric fHBP can optionally be immobilized on a support through covalent or noncovalent attachment.
Compositions of the invention include modified fHBP sequences.
The reactive residue in fHBP was from the strain used as the source for immunization.
A more comprehensive vaccine could combine both fHbp and ZnuD.
Multiple fHBP sequences are known and these can readily be aligned using standard methods.
Ideally it includes equal masses of the two different fHbp polypeptides.
Exemplary chimeric fHBP are provided in the Examples below.
The panel included strains from each of the three fHBP families.
Reference herein to a fHbp may refer to the factor H binding protein from Neisseria meningitidis.
Generally bacterial host cells and yeast are of particular interest for chimeric fHBP production.
For expression of a chimeric fHBP of interest, an expression cassette may be employed.
Further antigenic components Compositions of the invention include polypeptides comprising modified fHBP sequences.
Binding of the mAbs to recombinant fHBP was measured by ELISA.
Thus the modifications effectively increase the cross-strain protection of fHBP.
See also
Surprisingly, the immunogenicity of fHbp appears to be different between mice and guinea-pigs.
In this bactericidal condition, no killing was observed with the delta fHbp strain.
In one embodiment, the chimeric fHBP can be isolated using metal chelate chromatography methods.
The second immunogenic amino acid sequence may comprise a wild-type or modified fHBP sequence.
Panel A, fHBP is partitioned into three domains indicated by various shades of gray.
In addition, the role of JAR mAb epitopes in fH binding by fHBP was investigated.
Previously, we found that a region of V2 fHbp is involved in its susceptibility to trypsin digestion.
Figure 8B shows the amino acid identities of different domains of fHBP.
In this invention at least one modified V2 fHbp may be used as an antigen.
Native fHBP usually contains an N-terminal cysteine to which a lipid moiety can be covalently attached.
The composition may elicit a bactericidal antibody response effective against bacterial strains expressing the V2 fHbp.
In order to do this V2 fHbp would need to be stabilised.
FHbp specifically binds human and some non-human primate FH proteins.
The stability of these V2 fHbp mutants was analysed.
All four mutants showed enhanced resistance to protease digestion compared to WT V2 fHbp.
The modified V2 fHbp may not be a chimeric protein.
FHbp ( factor H binding protein ) The fHbp antigen has been characterised in detail.
An immunogenic composition comprising a modified V2 fHbp according to any preceding claim.
The modified V2 fHbp may not have beta barrels from different fHbp variants.
Panel B, an alternative nomenclature describes the fHBP as having two structural domains.
Chimeric fHBP include those having a B domain that expresses an epitope bound by the JAR 5 mAb.
The remaining strains express subvariants of fHBP v.1.
Thus, the chimeric fHBP can be lipidated or non-lipidated.
Reference 21 discloses vesicles in which fHBP is upregulated.
Figure 5A - V2 fHbp with single and double amino acid substitutions.
Numbering in this figure is based on fHBP from strain MC58.
In some embodiments a bacterium over-expresses NHBA, NadA and fHbp.
Reference 15 describes various further forms of fHBP which are modified to improve their inter-family coverage.
ELISA was used to assess the response against both ZnuD and fHbp ( Table 3 ).
In some embodiments fHBP protein ( s ) will be lipidated e.g. at a N-terminus cysteine.
Figure 5 - illustrates the effect of stabilising mutations in V2 fHbp.
A full-length B domain of a v.1 fHBP generally is about 64 residues in length.
Residues GEHT are underlined followed by the second a helix ( AH2 ) of fHBP.
SEQ ID NO, 77 in reference 77 is another useful fHbp sequence which can be used.
The further antigens may include one or more of a V1 fHbp and a V3 fHbp.
