Examples of 'ficoll gradient' in a sentence
Meaning of "ficoll gradient"
ficoll gradient: A laboratory technique used to separate particles based on their density using a Ficoll solution
How to use "ficoll gradient" in a sentence
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ficoll gradient
Buffy coats are sometimes isolated by utilizing a ficoll gradient.
Monocytes are isolated in a Ficoll gradient then purified by adherence selection.
The technique used was isolation on a Ficoll gradient.
Ficoll Gradient Centrifugation Ficoll gradient centrifugation was used to remove dead cells.
Load blood cells onto a Ficoll gradient.
In each experiment, a Ficoll gradient was used to generate a population of PBMCs.
The PBMCs are purified on a Ficoll gradient.
The MNC were isolated by Ficoll gradient centrifugation at +18 days after the boost.
Defibrinated blood from volunteers was separated on a Ficoll gradient.
B and T lymphocytes were separated after Ficoll gradient centrifugation of rosetted lymphocytes.
Lymphocytes were isolated from peripheral blood using Ficoll gradient.
Human B lymphocytes are purified by Ficoll gradient from a pouch provided by LFB.
Blood was collected from healthy donors and PBMCs were isolated using a Ficoll gradient.
The sample may also be subjected to a Ficoll gradient prior to centrifugation.
PBMC were isolated from allergic volunteers by separation on a Ficoll gradient.
See also
For that, bone marrow was subjected to a ficoll gradient to remove non-nucleated cells.
Methods Human granulocytes were isolated from human blood by a Ficoll gradient.
The native PBMC 's were used directly after the Ficoll gradient centrifugation without the stimulation procedure.
PBMC were separated from whole blood using Ficoll gradient.
Leukocytes were isolated from whole blood by Ficoll gradient ( Böyum REF ) centrifugation.
Subsequently, the obtained cell suspensions were stratified and centrifuged on Ficoll gradient.
Mononuclear cells were isolated by Ficoll gradient centrifugation ( GE Healthcare ).
At was submitted to collagenase digestion, ucb and bm to ficoll gradient.
The PBMCs were isolated by centrifugation in Ficoll gradient ( Amersham Biosciences, Piscataway, NJ ).
Prior to stimulation, cell cultures were enriched for live cells by centrifugation through a Ficoll gradient.
Peripheral blood cells were purified over a ficoll gradient ( Lymphoprep ; Nycomed, Oslo, Norway ).
Exemplary enrichment techniques are immunoaffinity column, immunoaffinity beads, centrifugation through a sucrose or Ficoll gradient.
Peripheral blood mononuclear cells ( PBMCs ) were separated on a Ficoll gradient ( Ficoll-Hypaque, Sigma-Aldrich ).
Next, blood from a human volunteer was collected and lymphocytes were isolated using a Ficoll gradient.
PBMC was isolated from whole blood by standard Ficoll gradient centrifugation.
Receive blood bag and sterilize it with 70 % alcohol Load blood cells onto a Ficoll gradient.
After 24h cultured cell mixtures are collected and applied to a Ficoll gradient to isolate viable cells.
After a 40 minute digestion period at 37°C, the islets are isolated on a discontinuous Ficoll gradient.
The cells are enriched vía a Ficoll gradient.
For PBMC preparation, whole blood cells were collected through Ficoll gradient.
To obtain isolated islets, run a ficoll gradient.
In summary, buffy coat cells were separated on a Ficoll gradient.
Peripheral blood mononuclear cells ( PBMCs ) were obtained by Ficoll gradient centrifugation.
Panel b, blood mononuclear cell subpopulations, obtained using standard Ficoll gradient separation.
Peripheral blood mononucleate cells ( PBMC ) were obtained by Ficoll gradient separation.
The isolated primary human cell yield after separation on the ficoll gradient was 1.2X109 cells.
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