Examples of 'fluorogenic substrate' in a sentence
Meaning of "fluorogenic substrate"
fluorogenic substrate - A compound that is used in laboratory settings to produce fluorescence as a result of a specific chemical reaction, often employed in biological and chemical assays
How to use "fluorogenic substrate" in a sentence
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fluorogenic substrate
Similarly a fluorogenic substrate or fluorogen refers to a substrate coupled to a fluorophore.
Unbound conjugates are removed by washing and a chromogenic or fluorogenic substrate is added.
If a fluorogenic substrate is applied platelet poor or platelet rich plasma can be analysed.
Read concentration of released DNA in chamber with fluorogenic substrate.
This fluorogenic substrate releases its fluorophore under the action of a specific enzyme.
Problem B can be circumvented by using a fluorogenic substrate but this introduces problem C.
This fluorogenic substrate is cleaved by ICE.
In a preferred embodiment, the substrate is a synthetic fluorogenic substrate.
In some assays, the fluorogenic substrate is converted to a fluorescent product.
In various embodiments, the substrate is a chromogenic or a fluorogenic substrate.
A specific example of a fluorogenic substrate of this type would be 4-methylumbelliferyl phosphate.
In various aspects, the substrate is a chromogenic or a fluorogenic substrate.
In some embodiments, a chromagenic or fluorogenic substrate is added to provide visualization of the antigen.
Reactions were initiated by addition of 20 µL FluCa buffer containing calcium chloride and fluorogenic substrate.
In the method, FXIa cleaves a specific fluorogenic substrate in the presence of calcium.
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The variants are assayed by droplet electro-coalescence with 2 pL droplets containing a trypsin fluorogenic substrate.
In particularly preferred embodiments, the fluorogenic substrate typically consists of a substrate conjugated to a fluorogen.
Assays are carried out in 96-well plates in the presence of a specific fluorogenic substrate.
A fresh mixture of fluorogenic substrate and CaCl2 was prepared before starting each experiment.
In particular, it is a chromogenic or fluorogenic substrate.
In some assays, the fluorogenic substrate is converted to a fluorescent product by B-lactamase activity.
Caspase-3 activation was assessed by cleavage of fluorogenic substrate DEVD-AFC.
One mg of fluorogenic substrate was dissolved in 100 µl of dimethyl formamide.
Caspase activation was monitored by the cleavage of specific caspase-3 fluorogenic substrate DEVD-AMC.
For detection of POD-conjugates fluorogenic substrate QuantaBlu ( Pierce ) was used according to manufacturer 's instructions.
Figure 2 illustrates the detection of B-galactosidase activity expressed by E . coli using the fluorogenic substrate MUG.
L-alanine-Mca, Ala-Mca, a fluorogenic substrate for measuring aminopeptidase activity was purchased from Sigma.
The substrate can be, e.g., a chromogenic substrate or a fluorogenic substrate.
This fluorogenic substrate is cleaved effectively by CPP32.
Fig 5, Effect of compound 1, 6 and 7 on amidolytic activity of human alpha-thrombin using fluorogenic substrate.
Both fluorogenic substrate peptides were obtained from Peptide Institute Inc. ( Osaka, Japan ).
The activity of R-gingipain was measured by the cleavage of the fluorogenic substrate Z-Phe-Arg-AMC • HCl ( Calbiochem ).
The starting reagent contains fluorogenic substrate and CaCl2 and automatically dispensed ( 20 µL per well ).
The residual CYP450 isozyme activity is assayed with a fluorogenic substrate for that CYP450 isozyme.
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Apply as a drench to the substrate surface at seeding
Substrate not supplied with the printer
Maybe the laminated substrate was the trigger