Examples of 'following transfection' in a sentence
Meaning of "following transfection"
following transfection - referring to the process of introducing foreign DNA into cells in biological research
How to use "following transfection" in a sentence
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following transfection
This is exemplified by the following transfection experiment.
The following transfection assay methods are provided by way of example only.
Complexes were not removed following transfection.
Establishing virus titers following transfection with a defective retrovirus vector including a selection gene.
The presence of recombinant virus was easily observed under the light microscope following transfection.
Following transfection cells were selected in puromycin and genomic DNA was prepared.
Once the culture were established the following transfection protocol was used.
Following transfection the plasmid DNA may become degraded or diluted by cell division.
Correct clones give CPE within one week following transfection.
One week following transfection full CPE had formed in the transfected flasks.
SVG cells which expressed tyrosine hydroxylase were identified in the cultures following transfection.
We monitored lactate content in culture supernatants following transfection of various retroviral envelopes and RBD.
Following transfection cells were selected in Blasticidin and genomic DNA was prepared.
Percentage of virus infected cells and titer of virus following transfection with RNA transcripts.
The following transfection mixture was then distributed in each well,.
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The three FX families were expressed following transfection in CHO.
Following transfection into cytotoxic hybridomas, expression of a novel functional T cell receptor was detected.
Importantly, neither agent affected the pattern of differentiation following transfection.
Virus progeny was harvested 5 to 7 days following transfection when cytopathic effects were evident.
A visual score of the transfected cells was obtained at 48 hours following transfection.
The following transfection mixture was prepared, for each well,.
Cells were analyzed by fluorescence microscopy and flow cytometry 48 hrs following transfection.
The portion of living cells following transfection in each case was about 95 %.
For constructs involving the metallothionein vector, expression was induced with CuSO4 following transfection.
No exon 46 skipping was detected following transfection with a random AON.
LacZ . These latter dishes were used to monitor LacZ expression two days following transfection.
Transfected cells were irradiated 36 hours following transfection and TNF was assayed 10 hours following irradiation.
The DNA / protein / lipofectamine complexes do not have to be removed following transfection.
QuantiGene ® analysis was performed at 24 hours following transfection to detect the level of mRNA down-regulation.
Silencing of co-transfected artificial luciferase target 24h following transfection.
Following transfection ICAM-1 deletion mutants were expressed in COS cells at characteristic broad levels ( Figure 5 ).
Cells were harvested and analysed 48 h following transfection.
Following transfection of the resulting plasmids, cellular RNA polymerase I transcribes an antigenomic ( uncapped ) RNA.
Cells were incubated for 24 hours following transfection.
Following transfection of the resulting plasmid, cellular RNA polymerase I transcribes an antigenomic ( uncapped ) RNA.
Highest production levels were at days 4 to 8 following transfection.
Following transfection of 293 cells with the expression plasmid for prorelaxin, pRK.
Passage 10 is the first pass following transfection.
Following transfection cells were incubated at 27°C.
Cell viability assays were performed at 72 hours following transfection.
Luciferase activity was ascertained following transfection of liposomal DNA pre-incubated in serum containing medium ( 15 % ).
Cell viability analysis was performed at 72 hours following transfection.
Following transfection by electroporation, the a-Gal A activity in COS-1 cell extracts was increased 6 to 7-fold.
Branched DNA mRNA assays were performed 24 hours following transfection.
Cell viability analysis was performed at 72 hours following transfection with a range of PLK-1 SNALP dosages.
Thereafter, recombinant E1-deleted adenoviruses are isolated and purified following transfection.
Analysis of Jurkat cell genomic DNA following transfection with TRC 1-2 meganucleases.
The shuttle vector and insert ( M1 PCR ) were ligated and amplified in E . coli following transfection.
The three FX families were expressed following transfection in HEK293S.
The transfected cells were biosnythetically labeled on day 3, 7 and 14 following transfection.
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Examples of using Transfection
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Magnetic nanoparticle transfection is the technical term
The transfection efficiency is tested at variable times
Several methods of cell transfection exist at present
Examples of using Following
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The following lamp caps may be selected
Set the clock by following these steps
The following general findings can be made
