Examples of 'forward and reverse primers' in a sentence
Meaning of "forward and reverse primers"
forward and reverse primers - In molecular biology, these are short, single-stranded nucleic acid sequences used in PCR (polymerase chain reaction) to amplify specific regions of DNA through complementary base pairing
How to use "forward and reverse primers" in a sentence
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forward and reverse primers
Thin lines represent forward and reverse primers.
Both forward and reverse primers are designed as described above.
Horizontal arrows indicate location of forward and reverse primers.
The various forward and reverse primers that were used are described below.
An oval indicate insertion of LNAs in forward and reverse primers.
The forward and reverse primers are colored dark and light gray respectively.
In certain embodiments the forward and reverse primers may be identical.
The forward and reverse primers each hybridize to a different sense or antisense strand.
The probe anneals to specific nucleotides between the forward and reverse primers.
Forward and reverse primers overlap each other according to the diagram in fig.
The probe sequences were designed based upon the forward and reverse primers used.
Useful sets include pairs of forward and reverse primers optionally teamed with sequencing primers.
This is because different colored dyes can be used for the forward and reverse primers.
Underlined are forward and reverse primers forthe SSR amplification.
The two capture primers contain sequences that correspond to the forward and reverse primers.
See also
The forward and reverse primers are designed to amplify all or part of a CRISPR region.
Positive clones will be identified and sequenced with the commercially available forward and reverse primers.
VH and VI forward and reverse primers were as described above.
Positive clones were amplified by PCR using universal λ forward and reverse primers.
The sequences for the forward and reverse primers for Fragment A was as follows.
PCR were performed with different to combinations of these forward and reverse primers.
Table 1 shows the forward and reverse primers used for each amplification.
The following is a list of the respective forward and reverse primers used,.
Preferably, the forward and reverse primers do not comprise overlapping nucleotide sequences.
For each region of the chromosome, a set of forward and reverse primers is designed.
The pin II forward and reverse primers were as follows,.
Pre-amplification of the cDNA can be accomplished using universal forward and reverse primers.
Once the nucleic acids are prepared, forward and reverse primers are introduced to the sample.
Most preferably, the first nanoparticles contain on their surface both forward and reverse primers.
In PCR, both forward and reverse primers anneal to the same fragment to enable amplification.
PCR 's containing all four possible combinations of forward and reverse primers were performed.
The forward and reverse primers used for SP2 distribution in different serotypes were respectively,.
In certain embodiments, both forward and reverse primers are tagged.
Both forward and reverse primers are used as illustrated in Figure 3A.
An exemplary process for selecting the appropriate forward and reverse primers is illustrated in FIG . 5.
During PCR, forward and reverse primers hybridize to a specific sequence of the target DNA.
More preferably, the nanoparticles are conjugated to the forward and reverse primers of the PCR.
Figure 2 shows forward and reverse primers for RT-PCR reactions to detect ALK fusions.
The 5 ' amyA flanking sequence was PCR amplified using forward and reverse primers shown below.
Forward and reverse primers used for regions resequenced in the PLEKHA1 and HTRA1 genes.
In a subsequent PCR, FLINT specific forward and reverse primers are used to amplify the cDNA.
An oval indicate insertion of Locked Nucleic Acids ( LNAs ) in forward and reverse primers.
The forward and reverse primers are,.
Purified plasmid DNA was sequenced in both directions using M13 forward and reverse primers.
The forward and reverse primers were chosen to produce amplicons of less than 500 bp.
Each selected plasmid was sequenced in both directions using M13 forward and reverse primers.
The SMN forward and reverse primers are used at final concentrations of 0.4 µM.
The target transcripts are amplified using two sequence-specific forward and reverse primers ( unlabeled ).
Non-limiting examples of forward and reverse primers are listed in TABLE 16.
Forward and reverse primers are identified by the suffixes “ . fwd ” and “ . rev ” respectively.
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For final sanding of primers before coating
Alkyd primers utilise corrosion protection pigments
Hybridisation of the primers with the template
Examples of using Reverse
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Proceed in reverse order to remove the accessory
This option does not work in reverse rotation
Reverse the procedure to reinstall the divider
