Examples of 'freezing medium' in a sentence
Meaning of "freezing medium"
freezing medium - A substance or environment that is extremely cold and used to preserve biological samples or other delicate materials
How to use "freezing medium" in a sentence
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freezing medium
Cells were resuspended in freezing medium and cryopreserved.
Resuspension is performed gently with cold freezing medium.
One mL of cells in freezing medium was transferred to each cryovial.
Virus is harvested by removing the medium and replacing it with freezing medium.
As a freezing medium.
Freezing the collagen cell carrier with adherent cells in a freezing medium.
The fibroblasts can be frozen in any freezing medium suitable for preserving fibroblasts.
Prior to sectioning, tissues were embedded in freezing medium.
According to the invention, this type of freezing medium can be implemented with chondrocytes.
Cells were collected by centrifugation, washed, and resuspended in freezing medium.
The pellet was frozen in OCT freezing medium to form a block.
In other embodiments, the dendritic cells are incubated after washing and resuspended in freezing medium.
Profreeze is sold by Lonza-BioWhittaker as freezing medium containing components of non-animal origin.
Freezing medium is specifically designed to allow for impact-free freezing of cells for long-term storage.
Here, the pellet of cells is re-suspended in freezing medium.
See also
The traditional freezing medium contains animal serum, mainly fetal calf serum and / or bovine calf serum.
Then, the cells were recovered and re-suspended in a freezing medium.
The freezing medium used was skim milk with 1 % glycerol.
After the incubation period, the transformed cells were pooled and mixed 1:1 with freezing medium.
The freezing medium generally consists of a diluter, ( sometimes ) a protein source, as well as a cryoprotectant compound.
The cells were thawed in 37 °C water bath and centrifuged, and the freezing medium was removed.
The freezing medium was 10 % DMSO in bovine serum.
The donor ES cells were frozen in freezing medium containing 10 % DMSO until use.
In the last incubation step, the medium on the capsules is 100 % freezing medium.
The results obtained for freezing medium 1 ( without DMSO ) are presented in Table 2.
For storage, cells can be frozed from one T75 flask into 4 cryotubes with 1 ml freezing medium.
Tissue freezing medium ( Ted Pella Inc., Redding, CA ) on dry ice and methylbutane.
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Examples of using Freezing
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Water freezing in the system will break it
It must not be used in freezing outdoor conditions
The freezing canister is filled with liquid