Examples of 'full-length cdna' in a sentence
Meaning of "full-length cdna"
Full-length cDNA (complementary DNA) is a complete copy of an organism's RNA (ribonucleic acid) sequence, which represents the entire set of genetic instructions for that organism. It is produced through a process called reverse transcription, where an enzyme synthesizes a DNA copy from the RNA template. Full-length cDNA is widely used in molecular biology research to study gene expression, protein function, and various biological processes
How to use "full-length cdna" in a sentence
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full-length cdna
Construction of the full-length cDNA clone.
Full-length cDNA clones were identified by sequencing.
Modification and sequence analysis of full-length cDNA clones.
A full-length cDNA clone was obtained and sequenced.
Several methods can be employed to obtain full-length cDNA constructs.
A human liver full-length cDNA library was constructed using standard techniques.
This probe was used to clone the corresponding full-length cDNA.
A mouse liver full-length cDNA library was constructed using standard techniques.
Both genes consisted of a cytomegalic virus promoter linked to a full-length cDNA.
A full-length cDNA clone may be obtained or constructed from overlapping fragments by standard procedures.
This was used for final determination of the full-length cDNA sequences.
A full-length cDNA was then isolated using the cDNA fragment, sequenced and identified.
To within five nucleotides of either end of the mRNA sequence full-length cDNA.
The bands at the top of the gel were full-length cDNA products in this assay.
A probable full-length cDNA sequence for this protein was recently deposited in the GenBank.
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Other methods employing amplification may also be employed to obtain a full-length cDNA sequence.
In this manner a full-length cDNA encoding the polypeptide of interest is isolated and sequenced.
One of the advantages of PCR-based methods is the ability to generate full-length cDNA.
A full-length cDNA molecule can be generated by ligating suitable fragments, using well known techniques.
Such methods are particularly effective in combination with a full-length cDNA construction methodology, above.
Full-length cDNA templates are generated by ligation of appropriate restriction fragments derived from these plasmids.
In optional embodiments, the cDNA library is constructed using a full-length cDNA synthesis method.
Full-length cDNA clones can be isolated vÃa nucleic add hybridization using for example cDNA libraries.
A number of cDNA synthesis protocols have been described which provide substantially pure full-length cDNA libraries.
A full-length cDNA sequence is assembled from the overlapping partial clones for each of the kinases.
In this case the DNA is a full-length cDNA copy.
Then, the human full-length cDNA was cloned from the clone isolated from the cDNA library.
PCR technology can also be utilized to isolate full-length cDNA sequences.
Transcripts of IV full-length cDNA lacking a cap structure were not infectious.
Targeting a complex transcriptome, The construction of the mouse full-length cDNA encyclopedia.
The full-length cDNA clone containing the SH deletion was designated pRSVASH.
The specificity of ribo-probe was identified by a Dot blot against full-length cDNA.
The full-length cDNA clone was isolated from a human leukocyte library by PCR.
These mutations were subsequently incorporated into a full-length cDNA clone to recover infectious PRRSV.
Hybridization of a full-length cDNA to an mRNA will protect the RNA from RNase degradation.
In this document we describe the generation of infectious NDV entirely from cloned full-length cDNA.
The inserted DNA contained full-length cDNA corresponding to structural gene mlcE.
A full-length cDNA was obtained using the RACE protocol of Frohman et al.
The inserted DNA contained full-length cDNA corresponding to structural gene mlcR.
Full-Length cDNA Clone Attempts to isolate a full-length clone from the original cDNA library failed.
PCR technology can also be used to isolate full-length cDNA sequences from other organisms.
Mouse MANF2 full-length cDNA in pCRII vector was linearized before synthesizing RNA probe.
Isolation and Assembly of a Full-Length cDNA for SPS.
Preferably, full-length cDNA clones are used for sequencing.
A cDNA according to claim 1 comprising a full-length cDNA.
Construction of Full-Length cDNA Clones of IBDV genome.
Considering the sequence, this cDNA is not considered to be a full-length cDNA.
Construction of full-length cDNA clones.
Only one full-length cDNA clone corresponding to the subtractive clone 16 was isolated.
The nucleotide sequence of the full-length cDNA was determined to be,.
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Examples of using Cdna
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
