Examples of 'geneticin' in a sentence
Meaning of "geneticin"
Geneticin is an adjective used in biology to refer to a type of antibiotic that is commonly used as a selective agent in cell culture
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- An aminoglycoside antibiotic that blocks polypeptide synthesis
How to use "geneticin" in a sentence
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geneticin
The geneticin serves as a selection marker.
The vector confers resistance to geneticin and ampicillin.
Geneticin resistant cells have been obtained.
Stable clones were obtained using geneticin antibiotic selection.
Geneticin was used as a positive control.
Stable transfectants were isolated by positive selection in medium containing geneticin.
The amount of geneticin used was based on a minimal lethal dose titration.
The expression of this protein is selected with the help of geneticin.
Geneticin preferentially destroys nontransfected cells in order to maintain a pure culture.
Stable lines were generated by selecting cells grown in culture media containing geneticin.
Geneticin resistant cells were obtained and these cells were further selected for resistance to methotrexate.
Transfected cells were maintained in culture media supplemented with an optimized concentration of geneticin.
The pMCN vector has a Geneticin resistance gene.
For each transfected cell line selection of stable transfectants was carried out with geneticin.
Geneticin was added to the transfected cells to maintain KSA expression.
See also
Each clone which has integrated the cassette is resistant to geneticin and forms a colony.
The level of Geneticin resistance depends on the number of kanamycin genes integrated.
It has a neomycin resistance marker for Geneticin selection.
Fresh Geneticin containing DMEM media was added every four days.
Cells were transfected using Lipofectamine and further selected using geneticin.
Geneticin and all restriction enzymes were obtained from Boehringer Mannheim.
Stably transfected EPO producing cells are selected according to their resistance to geneticin.
CAT fluorescence and Geneticin resistance were employed to select permanently transformed cell lines.
All plants that formed actively growing roots on geneticin also fluoresced Fig.
Thus, not every geneticin resistant colony is a true transformant.
Positive clones are selected with the selection antibiotic geneticin at 1 mg / mL.
Clones which were resistant to geneticin were isolated from the 5 transformations which were carried out.
The next day, the cells are selected in the presence of geneticin or phleomycin.
Throughout Geneticin selection, media was changed approximately every 3 to 5 days.
An example is the kanamycin or geneticin resistance gene, nptII.
Three days after transfection, the transformed cells are selected in the presence of geneticin.
An example is the kanamycin or geneticin resistance gene, nptll.
Drug-resistant cells were selected in the presence of Geneticin.
Geneticin resistant clones were selected 10-14 days after selective media was added.
Transfectants producing Ig-INSB were selected in the presence of geneticin and mycophenolic acid.
Geneticin resistant ( genr ) colonies were counted at 14 to 16 days after beginning selection . CFTR complementation.
Stably transfected cells were selected by culturing in Schneider 's medium containing geneticin.
Fibroblast contamination was suppressed by adding geneticin ( 250 micrograms / ml ) to the medium for 2 days.
The pPIC9K vector contains the kanamycin gene that confers resistance to Geneticin in Pichia.
The latter imparts resistance to the aminoglycoside antibiotics, such as kanamycin, neomycin, and geneticin.
After 2 days for phenotypic expression the selective agent geneticin was added at 400 ug / ml.
Subsequently, transfected cells were selected in the above medium supplemented with 400µg/mL geneticin.
Medium was changed every 2 or 3 days to maintain Geneticin potency.
Stably transfected cells were selected for 1 week in growth medium containing 1mg . cm-3 Geneticin.
Cells were grown under continuous selection pressure by the addition of 800 µg / mL geneticin GIBCO-Invitrogen Corp.
Single-cell clones of the parent cell line were selected by growth in 10 % APK9 and Geneticin.
HBL100 cells were transfected with a pSUPER BRCA1 RNAi plasmid and selected with geneticin for 3 weeks.
CHO DHFR - cells are co-transfected with modified pcDNA3.3 and pOptivec under selection from Geneticin.
The transduced cells were cultured in the same medium supplemented with Geneticin ( 1 mg / mL ).
The pSV2neo contains a selectable marker gene conferring resistance to the antibiotic geneticin ( G418 ).
