Examples of 'guanidine hydrochloride' in a sentence
Meaning of "guanidine hydrochloride"
a chemical compound commonly used in biochemistry for protein denaturation. This phrase is used in scientific contexts to refer to a specific chemical compound
How to use "guanidine hydrochloride" in a sentence
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guanidine hydrochloride
Preferred denaturants are guanidine hydrochloride and urea.
Guanidine hydrochloride is very expensive and must be replaced for the naturation process to occur.
A suitable example is guanidine hydrochloride.
Guanidine hydrochloride was from Fluka.
In a further preferred embodiment said denaturant is guanidine hydrochloride or urea.
Inclusion bodies were resuspended in guanidine hydrochloride containing dithiothreitol and solubilized at room temperature.
The relaxin polyeptide may be solubilized with urea or guanidine hydrochloride.
Preferred methods for removing the guanidine hydrochloride include diafiltration and dialysis.
The reaction mixture may contain a denaturant such as guanidine hydrochloride.
The relationship between guanidine hydrochloride concentration and peak wavelength was compared among the antibodies.
Polyalkylene guanidines are copolymers obtained by condensation of guanidine hydrochloride with an alkylenediamine.
In certain embodiments, guanidine hydrochloride or urea are the chaotropic agents.
The sample chamber is preloaded with a chemical lysis reagent guanidine hydrochloride.
This kit uses a column of guanidine hydrochloride in order to retain the DNA.
The most commonly employed strong denaturant in schemes of this type has been guanidine hydrochloride.
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Additives to the sol medium such as guanidine hydrochloride or urea are useful.
The combined aqueous phases from the wash step contain cyclohexyl tetraethyl guanidine hydrochloride.
Character B shows the effect of additional guanidine hydrochloride which causes dispersed analyte aggregates.
It can be released upon denaturation with 2-mercaptoethanol or guanidine hydrochloride.
Then guanidine hydrochloride and comonomer ( if present ) were added to the flask.
The reaction of known cyclopentyl guanidine hydrochloride Org.
Guanidine hydrochloride ( GuHCl ) - induced unfolding and refolding reactions were followed by measuring tryptophan fluorescence.
Chaotropic agents include for example urea, guanidine hydrochloride.
In brief, the cocktail is prepared by guanidine hydrochloride protein extraction of demineralized bone particles.
In one of the embodiments, the chaotropic auxiliary is urea or guanidine hydrochloride.
Next, the precipitate is treated with guanidine hydrochloride to dissolve the target polypeptide.
A process as claimed in claim 1, wherein the guanidine salt is guanidine hydrochloride.
Each bead was then washed in 8M guanidine hydrochloride to remove the bound streptavidin enzyme conjugate.
The method of any of claims 1 to 7 wherein said denaturant is guanidine hydrochloride or urea.
Generally 4 M guanidine hydrochloride solution may be used.
Suitable denaturing agents include, for example, guanidine hydrochloride and urea.
A guanidine hydrochloride alcohol-free binding buffer;.
Examples of chaotropic agents are urea, guanidine hydrochloride and ammonium thiocyanate.
Suitable salts include chaotropes of guanidine, such as guanidine carbonate or guanidine hydrochloride.
The final concentration of guanidine hydrochloride reached 5M.
Suitable examples of the nitrogen-containing compounds are urea, arginine and guanidine hydrochloride.
Guanidine hydrochloride or urea ; or a detergent e . g.
Alternatively, the lactoferrin receptor protein can be eluted by increasing concentrations of guanidine hydrochloride.
The remaining beads were then treated with 8M guanidine hydrochloride to remove any bound protein.
Each well was washed three times with approximately 300 µL of PBS to remove guanidine hydrochloride.
The crosslinked complex was then denatured in 8 M guanidine hydrochloride GuHCl and reduced and alkylated.
Guanidine hydrochloride ( Gdn HCl ) was ultrapure from Schwartz / Mann.
In some embodiments, an alcohol-free binding buffer comprising guanidine hydrochloride finds use in the technology.
In some instances 6M guanidine hydrochloride ( Sigma ) was used instead of urea for these steps.
The aggregated proteins were solubilized in 6M guanidine hydrochloride.
The factors used were protein concentration, guanidine hydrochloride concentration, pH, temperature, and arginine concentration.
CD-detected protein denaturation is provided as a function of the concentration of guanidine hydrochloride in Figs.
A recommended concentration of guanidine hydrochloride is about 4 to about 8 M.
The process of claims 2-21 wherein the denaturant used in step f is guanidine hydrochloride.
The solution was extensively dialysed against 4 M guanidine hydrochloride pH 4.0 in order to remove dithiothreitol.
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