Examples of 'high stringency' in a sentence
Meaning of "high stringency"
high stringency - refers to a strict and rigorous condition or standard that needs to be met, often used in scientific research or experimental procedures to ensure accuracy and reliability
How to use "high stringency" in a sentence
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high stringency
Hybridization is carried out at high stringency conditions.
Or very high stringency conditions.
Procedures using such conditions of high stringency are as follows.
Usually high stringency conditions are desirable in order to prevent false positives.
Stringent hybridization conditions can be of medium or high stringency.
Examples of moderate to high stringency conditions are provided herein.
Hybridization may be carried out under conditions of high stringency.
Examples of moderate and high stringency conditions are provided herein.
Suitable stringency conditions notably comprise conditions of high stringency.
Conditions of high stringency include higher temperatures of hybridization and washing.
The blot was washed at high stringency.
Under high stringency conditions only complementary nucleic acid hybrids are formed.
Preferred hybridising molecules hybridise under conditions of moderate or high stringency.
The conditions ensuring high stringency are well known to the skilled person.
The hybridization washing conditions used are preferably of a medium to high stringency.
See also
High stringency conditions vary with probe length and base composition.
All of the foregoing hybridizations would be considered to be under conditions of high stringency.
Moderate and high stringency hybridization conditions are well known in the art.
It is desirable to have probes which hybridize only under conditions of high stringency.
Other conditions of high stringency that may be used are well known in the art.
The filters were hybridized and subsequently washed under high stringency conditions.
Other conditions of high stringency which may be used are well known in the art.
Selective hybridisation may typically be achieved using conditions of medium to high stringency.
Filters were hybridized and washed under high stringency conditions using standard methods.
These high stringency hybridization conditions may be adapted by a person skilled in the art.
Only highly complementary nucleic acid hybrids form under conditions of high stringency.
Moderate or high stringency conditions will usually only allow a small proportion of mismatches.
Such sequences are substantially identical when they will hybridize under high stringency conditions.
Moderate and high stringency conditions can optionally be employed for sequences of greater identity.
The elevated reaction temperatures permit the ligation reaction to be conducted with high stringency.
A high stringency wash can be preceded by a low stringency wash to remove background probe signal.
Polynucleotides which hybridise at high stringency are included within the scope of the claimed invention.
High stringency hybridization conditions are those which only allow hybridization of substantially complementary nucleic acids.
Such primers and probes hybridize specifically to a target sequence under high stringency hybridization conditions.
High stringency hybridization conditions as for instance described in Sambrook et al.
Such probes and primers hybridize specifically to a target molecule under high stringency hybridization conditions.
Exemplary high stringency conditions are provided in the working Examples.
The nucleotide sequence which hybridizes under stringent conditions may hybridize under high stringency conditions.
The oligonucleotides used at high stringency are indicated in the Table below.
High stringency wash conditions were identical to those used for Northern blots.
For initial cycles of the PCR high stringency is so obtained.
Exemplary high stringency conditions are employed in the Examples presented below.
Positive clones were screened by means of the SST probe under high stringency.
More preferably, high stringency conditions are used.
In general, selective hybridization occurs at conditions of high stringency.
Alternatively, high stringency conditions can be used.
In an embodiment, the hybridization is under high stringency conditions.
Alternatively, high stringency conditions may be applied.
Post-hybridization washes were carried out at high stringency.
However, it is preferred to use high stringency hybridisation conditions.
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