Examples of 'his-tag' in a sentence
Meaning of "his-tag"
His-tag is a term commonly used in biochemistry and molecular biology to indicate a specific amino acid sequence added to a protein for purification and identification purposes
How to use "his-tag" in a sentence
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his-tag
Proteins bearing a His-Tag were purified from the supernatant.
The expression construct will be made with and without the HIS-tag.
The recombinant protein was expressed as a His-Tag fusión protein with a signal peptide.
His-tag purification is then performed followed by flag-tag purification to obtain interacting particles.
For protein purification the hypoallergenic molecule according to, this document is fused to a his-tag.
His-tag purification of a randomly chosen ankyrin repeat protein generated according to the present invention.
The polypeptide was expressed as a His-tag polypeptide.
His-tag purification under denaturing conditions including refolding of the repeat proteins in the purification column.
Immunoblot using antibody against the His-tag of gH.
The His-tag was removed by thrombin cleavage.
In some cases such polypeptide does not include a His-tag.
The His-tag mutant versions were also generated.
Purification is facilitated if the His-tag is used during purification.
The His-tag facilitates polypeptide purification and identification.
Preferably each monomer unit does not include a His-tag.
See also
Thus no His-tag would be present in the protein product.
The heterodimers were constructed with a His-tag for purification purposes.
His-tag protein was purified by affinity chromatography to a Ni-NTA Superflow column.
Preferably such amino acid sequence does not include a His-tag.
Said His-tag comprises or consists of six histidine residues in length.
Accordingly, bispecific antibodies which do not comprise a His-tag are alternatively preferred.
A His-tag fused recombinant protein can be produced using the vector.
A preferred embodiment of the invention is wherein the affinity tag is a His-tag.
Usage of this vector enables production of a His-tag fusion recombinant protein.
Similar results were also obtained with the constructs lacking a His-tag.
Usage of this vector enables production of a His-tag fusión recombinant protein.
But facultatively there are few additional amino acids comprised in the His-tag.
The hydrophobicity of the His-tag may also be enhanced by including hydrophobic residues.
A bispecific antibody of the invention may also comprise a His-tag as a heterologus poypeptide.
The His-tag in the proteins enables purification with immobilized metal affinity chromatography.
All proteins were expressed with a C-terminal His-Tag to facilitate isolation of the polypeptide.
A variant of NTA is used for protein isolation and purification in the His-tag method.
In a preferred embodiment, the tag is a His-tag and the surface substrate is nickel.
The protein produced by the host cells has been purified with a Ni-column through a His-Tag.
The additional His-tag and carboxyl amino acids encoding region are shown in bold . FIG.
The probability of inactivation is further decreased because the His-tag functions as a spacer.
In a His-tag sequence the Histidines represent the essential portion.
Only the region corresponding to the sSortilin construct is shown with the C-terminal His-tag omitted.
The His-tag and the Factor Xa cleavage site are shown entirely.
The extracted enzyme from Sf9 cells was partially purified using His-tag affinity column.
The cleaved His-Tag was separated from the tTF by anion-exchange chromatography.
All plasmids carried the synthetic linkers in frame with the His-tag and GFP.
In this fragment, the his-tag was not translated.
In certain embodiments, the polypeptides comprise a HIS-Tag.
Alternatively the protein can be recombinantly expressed with a HIS-Tag and purified using Ni2+/NTA chromatography.
In certain embodiments, the polypeptides do not comprise a HIS-Tag.
An additional N-terminal his-tag is present on the fusión proteins.
All variants contained a HIS-tag.
Fn3 and monobodies with His-tag were purified as follows.
The probes may include one or more tags, preferably selected from his-tag and PS-tags.
