Examples of 'homology arms' in a sentence
Meaning of "homology arms"
homology arms - In genetics and molecular biology, homology arms refer to regions of DNA sequences that share similarity or identity and are used in techniques such as PCR or gene editing for targeted DNA manipulation
How to use "homology arms" in a sentence
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homology arms
Homologous arms are also referred to as homology arms.
The homology arms on each side can vary in length.
Homologous recombination is promoted by providing homology arms on the donor DNA.
Left and right homology arms are shown at the ends of the construct representation.
Cells are then re-transformed with the Kanomycin PCR fragment containing homology arms.
The sequences of the two homology arms are chosen according to the experimental design.
Arm-specific assays determine copy numbers of a DNA template in LTVEC homology arms.
For ease of reference the homology arms include an upstream and a downstream homology arm.
Each terminus of the linear vector consisted of a BstZ17I site and 2 homology arms.
The homology arms allow for homologus recombination of the vector with the host genome.
In a specific instance, both homology arms are mouse homology arms.
AAVS homology arms are shown underlined and emboldened.
In a specific embodiment, both homology arms are mouse homology arms.
The two homology arms are chosen to flank the target DNA.
The first set was designed to amplify a region with the homology arms of AAV412.
See also
It has left and right homology arms as presented in figure 3.
Short homology arms were necessary due to limited packaging capacity of self-complementary AAV.
In still other embodiments, the homology arms are derived from a synthetic DNA.
The nucleic acid inserts are flanked by 5 ' and 3 ' homology arms.
Alternatively, the homology arms can be from a related genome.
In such methods, the nucleic acid inserts are flanked by 5 ' and 3 ' homology arms.
Alternatively, the homology arms can correspond to a region on a targeting vector in a cell.
Alternatively, the exogenous donor sequence can comprise 5 ′ and 3 ′ homology arms with no nucleic acid insert.
In some cases, the homology arms are derived from synthetic DNA.
Figure 31 shows the sequences of ROSA26 left and right homology arms.
In one embodiment, the homology arms are derived from a synthetic DNA.
Figure 1A depicts pictorially the meaning of ' inside ' and ' outside ' of the homology arms.
However, homology arms of 30 or greater residues increase efficiency.
In some targeting vectors, the 5 ' and 3 ' homology arms correspond to a targeted genome.
Extra-long homology arms did not improve gene targeting efficiency ( XX versus II ).
Some targeting vectors comprise 5 ′ and 3 ′ homology arms with no nucleic acid insert.
On the other hand, the target DNA is, by definition, inserted ' inside ' the homology arms.
For ease of reference the homology arms are referred to herein as a 5 ' and a 3 ' homology arm.
The method of claim 24, wherein said LTVEC comprises homology arms that total greater than 20 kb.
In some cases, the homology arms can also function as a 5 ' or a 3 ' target sequence.
In some targeting vectors, the 5 ′ and 3 ′ homology arms correspond to a safe harbor locus.
In one embodiment, homology arms are homologous to non-human CD8a or CD8B sequence.
A large targeting vector ( LTVEC ) containing homology arms and human IL-15 gene sequences was made.
As in Figure 1, the homology arms are shown in thick grey blocks.
In one disclosure, the non-human homology arms are homologous to a non-human B2 microglobulin locus.
Donor polynucleotides comprise homology arms flanking the insertion sequence ( e.g., DSBs in the DNA ).
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Examples of using Arms
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Just be sure the arms you are in are mine
Her arms are crossed over his breast
I held you in my arms when you were born
Examples of using Homology
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The homology of leaves is an easy conclusion to make
There may be partial homology or complete homology
A homology domain is generally longer than motifs
