Examples of 'hpai' in a sentence
Meaning of "hpai"
hpai (noun) - stands for Highly Pathogenic Avian Influenza, a term used in veterinary science to describe a contagious and severe form of avian flu in birds
How to use "hpai" in a sentence
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hpai
Decision on the highly pathogenic avian influenza hpai.
The HpaI site can be used to clone blunt ended fragments.
This plasmid was linearized with HpaI and XbaI linkers were ligated to the ends.
Ligation of the coupler to the blunt end resulted in destruction of the HpaI site.
The linearized plasmid is digested with HpaI and the vector fragment recovered.
Hpai and Xhol restriction sites are shown in bold.
The DNA was finally digested with HpaI and religated.
The HpaI deletion was evaluated both in an S . typhimurium background and in the S. typhi background.
The reverse primer incorporated the Hpai restriction site.
Plasmid pJDB207 is digested with the restriction enzymes KpnI and HpaI.
The forward primer incorporated an Hpai restriction site and a RBS.
The two PCR fragments were ligated together using the HpaI site.
Furthermore, digestion of the vector with HpaI results in a blunt-ended fragment.
The PCR products were treated with HpaI.
The stop codon is in bold ; the HpaI site used for cloning is in italics.
See also
The restriction sites introduced are NotI, HpaI and PacI.
Plasmid pJLK07 was digested with AvrII and HpaI and ligated with the annealed oligonucleotides.
The basic construct pM58 was digested with restriction enzymes HpaI and BalI.
The PCR fragment digested with HpaI replaced the NarI-HpaI fragment in pAeFl.
This fragment does not contain the restriction sites XbaI, ClaI, HpaI and SphI.
The gene is located between the Hpai and EcoRl restriction sites ( see Figure 5 ).
The plasmid pPL-lambda ( Pharmacia Co. ) was digested with the restriction endonucleases EcoRI and HpaI.
The 5 ' oligonucleotide also contains a HpaI restriction site at its 5 ' end.
An enhancer-like sequence from the 35S CaMV promoter is contained between the BamHI and HpaI sites.
Plasmid pJJ3942 contains HindIII, BamHI, and HpaI as unique cloning sites near the right border.
Plasmid pJDB207 is linearized by cutting with HpaI.
Plasmid pDP31 ( Example 9 ) is digested with PstI and HpaI resulting in three fragments.
Nucelotide 1 of M13KO7 is the third residue of the unique HpaI restriction site.
PCIStPA also was cut with Clal and Hpai and a 5340bp fragment isolated.
The pLXPCMTV backbone was digested with the restriction enzyme HpaI yielding a 7800 bp fragment.
This fragment sub-population was then ligated to the HpaI - XmaI sites of polink 26.
