Examples of 'hybridization buffer' in a sentence
Meaning of "hybridization buffer"
hybridization buffer: This phrase is commonly used in biology and molecular biology to refer to a solution that provides optimal conditions for hybridization reactions between nucleic acids. It helps facilitate the binding of complementary DNA or RNA strands
How to use "hybridization buffer" in a sentence
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hybridization buffer
The revealing probe is free in the hybridization buffer.
A hybridization buffer is also provided.
Each spot was afterwards analysed with a hybridization buffer.
In situ hybridization buffer.
The white blood cell pellet is resuspended in a hybridization buffer and counted.
The hybridization buffer used is determined by the nature of the probe used.
Each filter was incubated in hybridization buffer with one of the labelled probes.
A reference sample is pipetted into the remaining two tubes with CodeSet and hybridization buffer.
One hundred microliters of hybridization buffer was pipetted into each well of the microtiter plate.
The probe and sample are thereafter combined in a hybridization buffer and incubated.
The Capture Hybridization Buffer was warmed to room temperature.
In one example, naphthol is dissolved in hybridization buffer.
The ionic concentration of the hybridization buffer also affects the stability of the hybrid.
The precipitate was dried and dissolved in 2x hybridization buffer.
The probes were then treated with hybridization buffer to block nonspecific nucleic acid binding sites.
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After 2 hours of prehybridization, the labelled probe was added to the hybridization buffer.
The composition of the hybridization buffer was modified as follows,.
Preferably, the probe is diluted in a probe diluent that also acts as a neutralizing hybridization buffer.
The hybridization buffer was replaced with 5 ml / filter of fresh hybridization buffer.
The above-described membrane was blocked using a hybridization buffer of the same constituents as in Example 4.
A preferred hybridization buffer and amplification buffer are described above in the description of Kit 1.
Hybridization is carried out for 16 h in the hybridization buffer described by Church and Gilbert Proc.
Following purification, the labeled nucleic acids are transferred to 400 ul of hybridization buffer.
Addition of 2 ml of hybridization buffer and of 0.2 ml of solution containing the detection probes.
The labeled nucleic acids are transferred without purification to 480 ul of hybridization buffer.
For analysing miR16, first a hybridization buffer was made which was free of biological matrix.
Hybridization buffer was then added until the solvent front had migrated about 2.5 cm.
The slides were cooled on ice, and 48 µl hybridization buffer were added per slide.
The probe is used in hybridization buffer at a concentration of 1x106 dpm per millilitre hybridization buffer.
The slides are cooled on ice, and 48 µl hybridization buffer are added per slide.
Hybridization buffer only was used as the fourth concentration of DNA ( 0 nM ).
After 12 hours of soaking, the substrate was removed and rinsed with the hybridization buffer.
After 5 minutes 200 µl hybridization buffer containing 2 pmol of the respective biotinylated capture probe was added.
The porous wafer is washed three times with hybridization buffer at 65°C.
The SSC 20X hybridization buffer is an example in common use.
Hybridization was performed at 55°C using the hybridization buffer included in the kit.
Subsequently the hybridization buffer is discarded and the filter shortly washed using 2xSSC ; 0,1 % SDS.
The biotin-labeled p53 target DNA fragments were added to the hybridization buffer.
The probe was added to the slides in hybridization buffer and incubated overnight at 55°C.
The stringent buffer is removed and the array is rinsed with 200 µl 1X MES hybridization buffer.
Thirty µL 2 × Agilent Hybridization buffer is added to each sample and mixed.
To stop fragmentation reaction 55 µl of 2x GEx Hybridization Buffer was added.
In another embodiment, the hybridization buffer consisting essentially of 2 M to 6 M GuHCL buffer is used.
Using 30 % formamide in the hybridization buffer.
For a negative control ( Chip A ) 10 µL of water was added to a chip containing 1X Hybridization buffer.
Prehybridization is effected in the hybridization buffer at 42 ° C. for at least 4 hours.
Wash with 3 times with 1 ml of 1X Hybridization buffer.
Hybridizations were performed at 63°C in hybridization buffer 10 X Denharts solution Maniatis supra, pg.
Hybridization was completed at 42°C in commercially supplied hybridization buffer ( Roche ) overnight.
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Examples of using Hybridization
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There was no evidence of hybridization in the wild population
The hybridization is carried out in closed loop
Method and machine for hybridization by refusion
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
