Examples of 'i restriction' in a sentence
Meaning of "i restriction"
The term 'restriction' refers to a limitation or condition that controls or regulates an activity, often used in various contexts such as rules, laws, or physical barriers
How to use "i restriction" in a sentence
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i restriction
It contains an EcoR I restriction site and a splice site.
In this embodiment the restriction marker site is preferably an EcoR I restriction site.
As a result of the ligation the Sal I restriction site in the vector will be eliminated.
This sequence is responsible for the presence of the Ase I restriction site.
Typical type II restriction enzymes differ from type I restriction enzymes in several ways.
Plasmids were screened for the presence of the extra Sal I restriction site.
The plasmid contains a pair of Sal I restriction sites located at the site of the deletion.
No Ear I restriction endonuclease sites such that insertion of sequences would produce a unique site.
The pgsA region of this fragment included a Pst I restriction enzyme site.
Type I restriction enzymes have methylase and ATP dependent nuclease activities in the same molecule.
These results suggest that the Sph I restriction system functions poorly at elevated temperature.
Plasmids of the bacterial clones were screened for the absence of the Sma I restriction site.
The plasmid contains a unique Xba I restriction site located at the site of the deletion.
Each synthetic oligonucleotide was then cut from the vector using Fok I restriction endonuclease.
The plasmid contains a unique Pst I restriction site located at the site of the deletion.
See also
EcoR I restriction map of cosmids was performed essentially as described in Sambrook et al.
This creates an additional BCl I restriction site.
A Nsi I restriction site was added between these two sequences.
This gave rise to a Cia I restriction cleavage site.
The resultant pCBAK expression vector contained only one Spe I restriction site.
Unique BamHI and Not I restriction enzymes were inserted for shRNA cloning.
The cloning vectors were cleaved with Sma I restriction endonuclease.
It contains a unique Xho I restriction enzyme site into which foreign DNA may be inserted.
The cDNA is then digested with the Xho I restriction enzyme.
It contains a pair of Sal I restriction enzyme sites into which foreign DNA may be inserted.
Determining whether the amplification product is susceptible to digestion by Taq I restriction enzyme ; and.
The Xho I restriction site at the 3 ' end of the cDNA had been previously introduced.
The grey box represents the Mly I restriction endonuclease site.
Digestion with Spe I restriction enzyme results in production of " sticky ends " at both termini.
An unphosphorylated single stranded linker containing a Pvu I restriction site was purchased from Boehringer Mannheim.
The BamH I / BsiWI I restriction sites are highlighted utilised in transfer of the variable light region.
For easy identification of mutant gene, Sac I restriction site was generated.
This generates a Not I restriction site at the 5 ' end of the protease B promoter.
Fragment 2 is extensively digested with Bgl II and Not I restriction enzymes prior to ligation.
Bam HI and Pst I restriction sites ( underlined ) were included for cloning purposes.
First stage template was removed by digestion with Dpn I restriction endonuclease ( Life Technologies ).
A Sal I restriction site was introduced into each V-region upstream of the initiation codon, ATG.
Before ligation, the protruding ends of the Asc I restriction site were blunted with Klenow polymerase.
The TRP 1 gene was separate from yeast replicators, and bounded by Cla I restriction sites.
For example an Ase I restriction site may be inserted, which comprises the stop codon TAA.
A P. haemolytica strain which harbors a mutation which abolishes expression of Pha I restriction endonuclease.
Spe I restriction sites are not present on the linear amplified pBR322 vector.
The underlined sequence of the primers indicates Bgl II and Sal I restriction sites, respectively.
The Xho I restriction site for cloning into the pComb2-3 vector is underlined.
The pCR 4 vector encoding the linker is cleaved with Bam HI + Sal I restriction enzymes.
A former Kpn I restriction site ( GGTACC ), underlined above, has been eliminated by this codon alteration.
LK8 expression cassette and pδneo vector have Sal I restriction enzyme recognition site.
An Nhe I restriction site was incorporated into both the 5 ′ and 3 ′ ends of the coding sequence.
Underlined are the Hind III and, respectively, BamH I restriction sites in the corresponding primer.
The 3 ' ( reverse ) primer contained sequences that introduced a stop codon followed by a Sal I restriction site,.
