Examples of 'immunoprecipitates' in a sentence
Meaning of "immunoprecipitates"
Immunoprecipitates (verb): The process of isolating a specific protein by using antibodies to precipitate it out of a solution, typically for further analysis in laboratory experiments
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- plural of immunoprecipitate
How to use "immunoprecipitates" in a sentence
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immunoprecipitates
The immunoprecipitates were washed extensively with lysis buffer.
No enzyme activity was recovered in the immunoprecipitates.
The radiolabeled immunoprecipitates were visualized by fluorography.
Note that Notch appears as a doublet as is typical for Notch in immunoprecipitates.
The immunoprecipitates were analyzed by western blots.
The three phosphoepitopes of tau were detected in immunoprecipitates using specific antibodies.
Immunoprecipitates were immunoblotted with antibodies to phosphotyrosine.
Presence of GAD in the immunoprecipitates was analyzed by Western blotting.
Immunoprecipitates were then denatured and transferred to nitrocellulose membranes.
Proteins were phosphorylated with ATM immunoprecipitates.
The resulting immunoprecipitates were processed as described herein below.
Solubilization was shown to be nonselective and 23 immunoprecipitates were observed reproducibly.
Immunoprecipitates of the receptor retain ability to phosphorylate the receptor.
Coprecipitation of Notch was detected in Delta immunoprecipitates from cotransfected cells and embryos.
The immunoprecipitates are recovered by centrifugation.
See also
Immunoprecipitations using these products were performed, with detection of the immunoprecipitates as described above.
The immunoprecipitates were used directly as a substrate in the IkBa phosphorylation assay.
The HER-erbB protein was readily detected in immunoprecipitates before and after methotrexate amplification.
The immunoprecipitates were washed twice with lysis buffer and twice with kinase buffer.
The resolution of normal cell proteins by interaction with homologous antisera yielded at least 35 immunoprecipitates.
The resulting immunoprecipitates were subjected to western blot analysis with the reciprocal antibody.
Similar level of Bad is observed in control or peptide-treated anti-Bad immunoprecipitates.
Radiolabelled proteins in immunoprecipitates formed with the specific antibodies were also analysed on polyacrylamide gels.
The casein was not present in the analysis tests of immunoprecipitates in the SDS-PAGE gel.
Immunoprecipitates were washed three times with lysis buffer and once with buffered saline.
Any non-labeled VEGF in the immunoprecipitates may be detected by other suitable means, e.
Immunoprecipitates were subjected to Western blotting with the indicated antibodies.
Moreover, several phosphotyrosine containing proteins were found in Shc immunoprecipitates from PYK2 overexpressing cells.
The immunoprecipitates were washed with IP washing buffer and analyzed by western blotting.
Figure 2 summarized the results of Western blot analysis of immunoprecipitates detected with anti cYc antibodies.
The immunoprecipitates were washed with IP washing buffer and then subjected to western blot analysis.
Figure 4A depicts autoradiographic images of electrophoretically separated immunoprecipitates of PKR kinase.
Immunoprecipitates were washed and phosphatase activity estimated as in B.
Following washing, the immunoprecipitates may be separated by SDS-PAGE.
Immunoprecipitates prepared using anti-PPARY sepharose conjugate.
Kinase assays were performed using immunoprecipitates of p38 and JNK.
The immunoprecipitates were washed three times with the RIPA buffer and once with PBS.
The presence of delta antigen in the immunoprecipitates was assayed by immunoblot analysis ( Fig . 3A ).
The immunoprecipitates were analysed by immunoblotting with anti-HA or anti-FLAG antibody.
This also was reflected in an in vitro kinase assay performed on the immunoprecipitates ( FIG . 2B ).
Immunoprecipitates were dissolved in SDS-containing buffer then loaded onto a polyacrylamide gel.
RNA was extracted from liver homogenates and Ago2 immunoprecipitates ( IPs ) using trizol reagent.
Immunoprecipitates were resolved by SDS-PAGE and visualized by autoradiography.
Like the wild type EGF receptor, the HER-erbB hybrid incorporated significant amounts of 32p in immunoprecipitates.
Immunoprecipitates prepared using immobilized anti-IRP are shown in Fig.
Fab 4D has a lower affinity and 3F4 immunoprecipitates very weakly indeed.
Immunoprecipitates were resolved by SDS-PAGE and transferred to nitrocellulose membranes.
In a reciprocal experiment, cyclin D1 and CDK2 were also detected in anti-PCNA immunoprecipitates.
Immunoprecipitates prepared using immobilized anti-IRB are shown in FIG.
Western blot demonstrating that Sdc4 immunoprecipitates with Wnt7a receptor Fzd7 in mammalian cells.
