Examples of 'in vitro transcription' in a sentence
Meaning of "in vitro transcription"
In vitro transcription is a laboratory technique where RNA is synthesized from a DNA template outside of a cell. It is commonly used in biotechnology and research to study gene expression
How to use "in vitro transcription" in a sentence
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in vitro transcription
The in vitro transcription method was as previously described.
M indicates a marker prepared by in vitro transcription of a synthetic template.
The in vitro transcription methodology is known to the skilled person.
Wherein polyribonucleotides are produced by in vitro transcription or chemical synthesis.
In vitro transcription can be made.
The template sequences need to be removed after in vitro transcription.
The in vitro transcription method is not limited to the foregoing.
Nucleic acids can be directly labeled vÃa an in vitro transcription or an amplification reaction.
A suitable in vitro transcription medium therefore optionally additionally comprises an RNase inhibitor.
An expression vector may be used in in vitro transcription and translation assays.
After an in vitro transcription he uses a lysate from rabbit reticulocytes for the translation.
The generated plasmids were then subjected to in vitro transcription to generate RNAs.
A number of suitable in vitro transcription and translation systems are commercially available.
Given are the designation and the sequence of mRNAs obtained by in vitro transcription.
Any appropriate in vitro transcription conditions known in the art can be used.
See also
Plasmids were linearized with BanHI for use as templates for in vitro transcription.
Potential transcription systems are in vitro transcription systems or cellular transcription systems etc.
The nucleic acid construct can also be synthesized using in vitro transcription.
Suitable in vitro reaction conditions for in vitro transcription cocktails are generally physiological conditions.
The final aptamer sequence identified can then be prepared chemically or by in vitro transcription.
Such inefficiency is also relevant to the in vitro transcription of alphavirus expression vectors.
Another difficulty has been the production of a complete and active mRNA vÃa in vitro transcription.
In vitro transcription was used to produce RNA probes.
Then the linearized plasmid was used as a template for in vitro transcription as described herein.
In vitro transcription with oligonucleotide templates was conducted as described by Milligan et al.
The polypeptide may be provided from the nucleic acid by in vitro transcription and translation.
Conventional in vitro transcription methods then can be used to synthesize the RNA.
Probe synthesis is performed using the PharMingen in vitro transcription kit provided in the kit.
In vitro transcription Templates were amplified from corresponding plasmid constructs and extracted from gels.
The dsRNA molecules useful for the invention may also be produced by in vitro transcription.
Wherein the RNA is obtained by in vitro transcription or chemical synthesis.
Effect of target site orientation on PNA mediated termination of in vitro transcription.
The desired temple for in vitro transcription is a CAR described herein.
A radioactive antisense RNA probe is synthesized by in vitro transcription.
The desired temple for in vitro transcription is a CAR of the present invention.
PCR products are then purified and used for in vitro transcription.
RNA may be obtained by in vitro transcription of an appropriate DNA template.
IVT vectors may be used in a standardized manner as template for in vitro transcription.
The control RNA spikes were produced by in vitro transcription of linearized plasmid templates.
Several approaches were described for increasing the production of RNA molecules by in vitro transcription.
The antisense probe was synthesized by in vitro transcription from a PCR product.
In vitro transcription for hepatits Delta RNA.
This concern is also relevant to the in vitro transcription of Sindbis expression vectors.
Full length RNA transcripts were prepared from the above cDNA clones by in vitro transcription.
The synthetic RNA produced from the in vitro transcription is isolated and purified.
Total RNA prepared from lymphoma and neuroblastoma samples was used for in vitro transcription.
MRNA may be generated by in vitro transcription from a DNA template.
SpeI linearized plasmid DNA was used as template for in vitro transcription.
In addition, an in vitro transcription system may be used.
Preparation of PCR fragments for in vitro transcription.
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