Examples of 'incubation buffer' in a sentence
Meaning of "incubation buffer"
Incubation buffer: A solution used in scientific experiments to provide optimal conditions for culturing or growing organisms, cells, or biochemical substances
How to use "incubation buffer" in a sentence
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incubation buffer
Ul of calibrator or sample to be assayed diluted in the incubation buffer.
Incubation buffer or.
The substances studied were dissolved in the incubation buffer.
Ul of incubation buffer.
The test substances were dissolved in the incubation buffer.
The pH of the incubation buffer is chosen to maintain a significant level of specific binding.
The compounds to be tested were put into solution in the incubation buffer.
Radioligand was diluted in incubation buffer in borosilicate glass vials and protected from light.
The test compounds are dissolved in dimethyl sulphoxide and then in incubation buffer.
The rinse solution was pooled with the incubation buffer before collecting the desired DNA fragments.
A KinExA assay was developed to measure very low levels of antibody in an incubation buffer.
The ALDEFLUOR incubation buffer contains an inhibitor of MDR effluence pumps.
The column material had been previously balanced with the incubation buffer.
Subtract the Incubation Buffer control from all standards and samples.
For blanc values the enzyme was omitted from the reaction and replaced by incubation buffer.
See also
This incubation buffer was oxygenated for 5 minutes before incubation.
The buffer was used both as the assay buffer and incubation buffer for the sample.
This incubation buffer was oxygenated during 5 minutes before incubation.
Shaded boxes represent the IDUA activity in the incubation buffer.
The filters are washed rapidly with ice-cold incubation buffer and radioactivity determined by scintillation counting.
Immunodetection of IDUA secreted by transgenic plants into the incubation buffer.
IDUA activity present in the incubation buffer collected at various times post-inducton was plotted.
The kit of claim 8, further comprising a blocking buffer and an incubation buffer.
IDUA activity in tissue extracts and incubation buffer from transgenic IDUA-9 plant tissue.
Tissue pellets were washed superficially with 2 x 6ml of ice cold incubation buffer.
B, incubation buffer as a control . pI markers are indicated on the left.
The filter paper was washed 4 times with the ice cooled incubation buffer.
Add 50 µl of the incubation buffer to each well using a semi-automatic pipette.
Following the incubation period, unbound components were removed by a washing step with incubation buffer.
Cells were resuspended in 100 ml incubation buffer and incubated with the second staining reagent.
A bis-tris buffer having the same pH as the iron-containing incubation buffer discussed above.
Incubation Buffer One vial containing 10 ml of incubation buffer.
B, The dependence on pH in the incubation buffer.
Incubation buffer was then added up to a volume of 100 µL.
Wash the plate ( s ) with cells twice in incubation buffer.
For determination of total binding, incubation buffer with [ 3H ] BK but without compound was used.
Filters were washed with 4 x 1 ml aliquots of ice-cold incubation buffer.
A final volume of 0.25 mL of incubation buffer per well was used.
The high-salt rinse solution is pooled with the high-salt incubation buffer.
The volume of incubation buffer is determined so as to obtain a concentration of 5 × 10 6 cells / ml.
These filters are rinsed 4 times with 5 ml of incubation buffer preserved in ice.
The incubation buffer was PBS and the final concentration of [ 3H ] nicotine was 5 nM.
The incubation was stopped by adding another 1,7 ml of incubation buffer to the samples.
The precipitate was suspended in the incubation buffer at 2 ⁻ ⁴ x 108 platelet / ml concentration, immediately before binding.
Samples of human serum or plasma were pre-diluted with incubation buffer ad 5 %.
Blocking and antibody incubation buffer is PBST +5 % milk.
After the homogenate has been thawed at room temperature, 15 volumes of incubation buffer are added.
After thorough washing with incubation buffer the cells were preincubated for 10 min at 37 °C before use.
The pellet thus obtained wa suspended in 100 volumes of an incubation buffer solution.
After the 65°C incubation, the incubation buffer is collected and the membrane rinsed with high-salt buffer.
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Examples of using Incubation
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Incubation is terminated when positive growth is observed
I would guess the incubation time to be minutes
Incubation period is dropping all the time
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
