Examples of 'is transfected' in a sentence
Meaning of "is transfected"
This phrase is used in molecular biology or genetic research to describe the process of introducing foreign genetic material into a cell through techniques such as genetic engineering or gene therapy. Transfection can lead to the expression of specific genes or modification of a cell's characteristics
How to use "is transfected" in a sentence
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is transfected
The humanization library is transfected into cells.
This construct is transfected into embryonic stem cells and recombinants selected in culture.
Optionally in accordance with any method the invention a cell is transfected in vitro.
Preferably the vector is transfected into cultured insect cells.
Following ligation the vector with the insert of interest is transfected into cells.
A selected host cell is transfected with the vector and cultured.
In some embodiments, the cell or embryo is transfected.
The packaging cell is transfected with the minimal vector construct to make a producer cell.
An initial population of cells is transfected with DNA.
Injected RNA is transfected into the liver of living mice.
The vector containing the DNA to be expressed is transfected into a suitable host cell.
The plasmid is transfected into CHO cells for stable expression.
In some embodiments, the cell is transfected.
The corresponding cDNA library is transfected into cells allowing high expression of the various cDNAs.
The method of claim 5, wherein the packaging vector is transfected into the cell.
See also
Plasmid DNA is transfected into the cells using a suitable transfection reagent.
DNA encoding a fusión of this invention is transfected into a host cell for expression.
If a cell is transfected with this genome, this leads to the generation of progeny virus.
DNA encoding a fusion of this invention is transfected into a host cell for expression.
The vector is transfected into a recombinant host for propagation, screening, and cloning.
The expression vector is transfected into PER.
The scFv library is transfected Into the recipient bacterial hosts using standard techniques.
In another embodiment, the recombinant baculovirus is transfected into a eukaryotic cell.
The ligated viral DNA is transfected into the infected cells using standard calcium phosphate transfection.
In another embodiment, said recombinant baculovirus is transfected into a eukaryotic cell.
The recombinant plasmid is transfected into CHO DUX cells using standard calcium phosphate methods.
In this system, the tetracycline-inducible cell line is transfected with two plasmids.
The chimeric DNA sequence is transfected into a host cell that expresses the fusión protein.
These procaryotic elements are expected to be non-functional after pVk is transfected into mammalian cells.
The plasmid is transfected into CHO cells.
In further embodiments, the transduced antigen-presenting cell is transfected with tumor cell mRNA.
In some aspects, a cell is transfected as it naturally occurs in a subject.
A cDNA expression library prepared from a known or suspected ligand source is transfected into COS-7 cells.
In some embodiments, a cell is transfected as it naturally occurs in a subject.
Is transfected with the expression vector of claim 4 ;.
Generally, a vector is transfected into a helper cell line.
Unlike pCMV-luc, pCMV-eGFP provides information concerning what percentage of cells is transfected.
In some embodiments, a cell that is transfected is taken from a subject.
The replicon is transfected into Huh-7 cells using standard methods such as electroporation.
The recombinant cassette-containing vector is transfected into an appropriate host cell.
Each siRNA is transfected 3 times at each of the doses tested.
In a particular embodiment, said cell or cell culture is transfected with a vector of the invention.
Preferably, RNA is transfected into cells by standard techniques.
To generate the packaged vector, the vector plasmid is transfected into the packaging cell line.
This clone is transfected into DHFR ⁻ CHO cells as described above.
Specifically, cDNA encoding pancreatic islet glucokinase is transfected into the multicloning site of pIRESpuro3.
Each dish is transfected with 15 µg of the expression vector.
To generate the packaged vector, the retroviral vector is transfected into the packaging cell line.
The nuclease pair is transfected to a human cell line, e.g. HeLa cell line.
To generate a recombinant retroviral particle, the retroviral vector is transfected into the packaging cell line.
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Examples of using Transfected
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Transfected with human hepatic cancer cells
Thereby successfully transfected cells can be selected
A transfected cell can also serve as a carrier
