Examples of 'isotype control antibody' in a sentence
Meaning of "isotype control antibody"
Isotype control antibody: In the field of immunology and biochemistry, an isotype control antibody is a type of antibody used as a control in experiments to determine nonspecific binding and background signal levels, ensuring the accuracy and reliability of results when studying target antibodies
How to use "isotype control antibody" in a sentence
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isotype control antibody
An isotype control antibody is used as a negative control.
The binding was compared to isotype control antibody.
Isotype control antibody did not show any binding activity.
The blocking was compared to isotype control antibody.
Staining with isotype control antibody is shown in the shaded curve.
Control feeders were stained with an isotype control antibody.
Staining with an isotype control antibody was always negative.
No binding was observed in presence of the isotype control antibody.
Either no antibody or an isotype control antibody was used as a negative control.
Positive percentage is detected according to overlay with the isotype control antibody.
Staining with isotype control antibody is represented by the shaded curve.
The darker histogram represents an isotype control antibody.
The isotype control antibody treatment had no effect on the hyperalgesia.
Samples stained with a mouse isotype control antibody were negative.
An isotype control antibody and secondary antibody alone were used as negative control.
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Both antibodies perform significantly better than the isotype control antibody.
Mouse nonspecific isotype control antibody was used to confirm the specificity of staining.
The negative control was performed under identical conditions with an isotype control antibody.
The isotype control antibody should be of the same class as the test antibody.
Treatments consist of intraperitoneal injections given twice per week of antibody or isotype control antibody.
The results from a matched isotype control antibody are indicated by the shaded área.
Background fluorescence was assessed with platelets labelled with the FITC conjugated isotype control antibody.
Mice injected with an isotype control antibody after the collagen injections progressively developed disease.
Panel B demonstrates a similar blot probed with an isotype control antibody.
Both BSA and a human isotype control antibody were used as negative controls.
In one embodiment, the control antibody is an isotype control antibody.
A toxin-conjugate isotype control antibody was used as a control.
In each experiment, one group of animals received an isotype control antibody.
An IgG1 isotype control antibody was used as a negative control.
The control set of mice was treated with mIgG2a isotype control antibody.
In contrast, an isotype control antibody did not trigger macrophage-mediated phagocytosis.
Figures 4a-c indicate the background level of staining with isotype control antibody.
The negative isotype control antibody does not inhibit the VEGF-mediated phosphorylation of pVEGFR2.
Samples were thin-sectioned and stained with anti-endosialin or isotype control antibody.
Assay negative control antibody: Isotype control antibody diluted in human serum pool.
A FoxP3 expression-negative control was prepared by staining with the isotype control antibody.
An isotype control antibody is also used as a negative control, at 1ug/ml.
No increase in mean fluorescence was observed with the isotype control antibody ( data not shown ).
An isotype control antibody ( Isotype ) was used as a negative control.
Binding of biotinylated anti-CLDN1 mAb occurred only in the presence of isotype control antibody.
Negative controls were isotype control antibody and, without the 1st antibody.
In contrast, cartilage from the mouse receiving the isotype control antibody Fig.
A mouse isotype control antibody ( Zymed ) is used for negative control.
Figure 3b demonstrates the Western blot obtained from membranes incubated with the isotype control antibody.
Addition of the isotype control antibody ( HuMab-KLH ) did not induce ADCC.
In these experiments, no lysis was observed with the isotype control antibody ( data not shown ).
An isotype control antibody that is non-specific for CD70 was used as a negative control.
Binding to GDF-8 was confirmed using human isotype control antibody.
All of the blocking Mabs and isotype control antibody were used at a concentration of 20 µg / ml.
Mice were treated with anti-RGMb antibody 9D1 or an irrelevant isotype control antibody.
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Examples of using Isotype
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Appropriate isotype controls were used in parallel samples
The antibodies employed are preferably of the isotype
Isotype matched antibodies served as a negative control
