Examples of 'kinase reaction' in a sentence
Meaning of "kinase reaction"
Kinase reaction is a type of chemical reaction that involves the enzyme kinase, which catalyzes the transfer of phosphate groups. This reaction is commonly seen in biological processes such as cell signaling and metabolism
How to use "kinase reaction" in a sentence
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kinase reaction
The tyrosine kinase reaction was conducted as follows.
Was added thereto to terminate the kinase reaction.
The kinase reaction buffer is prepared essentially as described above.
Radioactively labelled by the well known kinase reaction.
After completion of the kinase reaction a binding buffer is added.
Required cofactors are added individually to each kinase reaction.
The kinase reaction was stopped with the addition of EDTA.
An important example of such a class of reaction is a kinase reaction.
The kinase reaction was performed on ice for 2 min.
There are two steps in the pyruvate kinase reaction in glycolysis.
The kinase reaction was started by the addition of 2 µl diluted enzyme.
The soluble fraction was used in the sphingosine kinase reaction.
The kinase reaction was carried out for 5 minutes at room temperature.
Labeled DNAs used in gel shift assays were prepared by Klenow extension or kinase reaction.
The substrate of the kinase reaction was an FITC-labeled SRCtide peptide included in the kit.
See also
For example, detection schemes can measure substrate depletion during the kinase reaction.
Following this, the kinase reaction is quenched, the substrate incubated in a second reaction with chymotrypsin.
In this assay, amount of ATP remaining in solution following a kinase reaction is measured.
The kinase reaction is stopped by washing the plates 6 times with TBST.
Prepare Gastrin and ATP working solution in kinase reaction buffer as follows,.
A Kinase reaction Buffer is prepared as follows,.
All the oligonucleotides are phosphorylated at the 5 ' end by a kinase reaction.
The kinase reaction is performed in a white 96 well microtitre plate as a Scintillation Proximity Assay.
The cells were then incubated for 24 hours before kinase reaction.
The kinase reaction is performed in a white 384 well microtitre plate.
Fluorescence intensity at 665 nm was used to monitor the extent of the kinase reaction.
The kinase reaction was terminated with 50 mM EDTA.
The method according to claim 7, wherein the step ( o ) is performed by a mevalonate kinase reaction.
The kinase reaction was stopped by adding EDTA to a concentration of 10 mM.
For hybridization, the DNA probes are labelled, e.g. radioactively labelled by kinase reaction.
The kinase reaction is initiated by the addition of ATF-2 and ATP mixture.
Addition of the p107 peptides to a p33cdk2 kinase reaction also inhibits the activity of the kinase.
The kinase reaction was initiated by the addition of a substrate cocktail containing MBP and Y-33P-ATP.
Key words, ER-docking, eukaryotic ribosomal protein, nuclear localization signal, recombinant ribosome, kinase reaction.
As substrate for the kinase reaction the peptide KKKSPGEYVNIEFG ( JPT, Germany ) is used.
To this mixture was added 10 µl of 100 µM ATP to initiate the kinase reaction.
The kinase reaction was incubated for 120 min. at room temperature.
Each mixture was incubated at 25°C for 60 minutes to perform a kinase reaction.
Stop the kinase reaction after 60 min by addition of 30 µL stop solution to each well.
The ATP concentration was 50 µM, and the kinase reaction was allowed to proceed for 16 h.
Stop the kinase reaction after 60 min by addition of 30 mL stop solution to each well.
The immunoprecipitates were washed and incubated with 10 mM B-mercaptoethanol in 150 µl of kinase reaction solution.
The non inhibited kinase reaction will result in a phosphorylation corresponding to 20%-30 % of the phosphorylation control.
Figure 9, Dose response curve for ATP, using 1 µM Alexa-peptide for the kinase reaction.
The kinase reaction was initiated by addition of ATP ( 1 mM final conc . ).
Specifically, the kinase reaction solution was added to a 96-well streptavidin-coated plate Product No.
The kinase reaction is initiated with 3 microliters of 40mM ATP.
As substrate for the kinase reaction biotinylated poly - ( Glu, Tyr ) ( Cis biointernational, France ) was used.
The kinase reaction was typically done with 100 µM ATP and 1 µM substrate.
The kinase reaction was done in 96-well half área black plate Greiner, Cat.
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Examples of using Kinase
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Creatine kinase can be checked if myalgias develop
Blood tests show elevated creatine kinase
Elevation of creatine kinase in blood is indicative of myositis
Examples of using Reaction
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It would give its reaction at a later date
Quick reaction times in case of delay s or malfunctioning
I am having a physical reaction to how bad this is
