Examples of 'klenow fragment' in a sentence
Meaning of "klenow fragment"
Klenow fragment: This phrase is a term in molecular biology referring to a DNA fragment generated by the enzymatic digestion of DNA. It is used in scientific research and genetic studies
How to use "klenow fragment" in a sentence
Basic
Advanced
klenow fragment
The fragment was filled in using Klenow fragment.
The Klenow fragment has several limitations when used for enzymatic sequencing.
Labeling of the end of the molecule with a Klenow fragment.
This fragment was treated with Klenow fragment before addition of XhoI linkers.
The XhoI end was made blunt by use of the Klenow fragment.
Overlaps were extended using the Klenow fragment and digested with HindIII and BamHI.
No difference in sensitivity was found between the holoenzyme and the Klenow fragment.
The cohesive ends were filled in using Klenow fragment as described earlier.
The remaining DNA fragment was digested with Bam HI and filled in with Klenow fragment.
These fragments are then biotinylated using Klenow fragment with standard procedures.
Prior to digestion with SstIId, the cohesive HindIII ends is made blunt-ended using Klenow fragment.
Three amplification reactions were done with the Klenow fragment using different pairs of primers.
The ends of the fragments were filled by using the DNA polymerase I Klenow fragment.
The excised neo gene was treated with Klenow fragment and a BamHl linker attached.
BRO3 was cut with XhoI and the ends were filled with Klenow fragment.
See also
The fragment was treated with Klenow fragment and ligated with Bcl I linkers.
The fragment was made blunt ended using Klenow fragment A.
The HIV-1 reverse transcriptase and the Klenow fragment have different behavior with respect to dUTP.
This form of the enzyme is called the exo - Klenow fragment.
Deoxynucleoside triphosphates and Klenow Fragment polymerase were the same as in Example 3.
The oligonucleotides were annealed and extended in a reaction using the Klenow fragment of DNA polymerase.
The 5 ' overhangs were filled in with Klenow fragment DNA Polymerase I and the plasmid was recircularised.
After digestion with Acclll, the purified DNA fragment is treated with Klenow fragment.
Extension reactions were carried out with the Klenow fragment of DNA polymerase as described.
Plasmid pCAH21 was first cleaved with BamHI and treated with DNA polymerase Klenow fragment.
The " sticky " ends are filled with PolA Klenow fragment in the presence of dNTPs.
The manual PCR uses a heat-labile DNA polymerase, such as DNA poll Klenow fragment.
PD neo was digested with EcoRI, treated with Klenow fragment and then a SalI linker attached.
The protruding Bglll and EcoRI ends are made blunt-ended by the polymerase I Klenow fragment.
DNA ends were filled-in with Klenow fragment and the plasmid religated.
Plasmid pTRKH4 was digested with Xba I and subsequently blunt ended with the Klenow fragment.
PDneo was digested with EcoRl, treated with Klenow fragment and then a Sall linker attached.
The Klenow fragment will fill in at 5 ' sticky ends.
The PCR product was treated with Klenow fragment and cyclized.
Subsequently, the Klenow fragment was heat inactivated by incubating the reaction mixture at 75°C for 5 minutes.
Each annealed group was extended with the Klenow fragment of DNA polymerase.
Figure 11 shows in a diagram the results of amplification reactions with the non-thermostable Klenow fragment.
The EcoRI overhangs are filled in with the Klenow fragment from E . coli DNA polymerase.
The Klenow fragment still retains the polymerase activity and the 3 ' - S ' exonuclease activity.
The single stranded ends were filled in with Klenow fragment and dNTP 's.
DNA polymerase, Klenow fragment was purchased from Boehringer-Mannheim.
Synthesis of cDNA using both reverse transcriptase and the Klenow fragment of DNA-Polymerase 1.
Furthermore, the Klenow fragment 29 offers the advantage that its optimal elongation temperature is at 37 °C.
The over-hanging ends were made blunt with the Klenow fragment of DNAPEcl and dNTPs.
In one embodiment, the Klenow fragment 29, which is not thermal-stable, is used as DNA polymerase.
The over-hanging ends were made blunt with the Klenow fragment of DNAPEc1 and dNTPs.
The Apal 5 ' protruding ends were made blunt by filling up with Klenow fragment.
The exo - Klenow fragment.
The ApaI 5 ′ protruding ends were made blunt by filling up with Klenow fragment.
The EcoRI sticky-ends were filled-in using the Klenow fragment of DNA polymerase I and dNTPs.
You'll also be interested in:
Examples of using Fragment
Show more
He has a fragment of sword embedded in his chest
There is a tiny missing fragment on the occipital
A small fragment impinging on her spinal cord
