Examples of 'laemmli' in a sentence
Meaning of "laemmli"
laemmli (adjective) - This term is used in biochemistry to describe a certain type of buffer solution that is used in electrophoresis. It refers to a specific chemical composition developed by Laemmli. For example, 'Prepare a Laemmli buffer solution for the electrophoresis experiment.'
How to use "laemmli" in a sentence
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laemmli
Laemmli lysis solution was added to the cells.
Cells were lysed in laemmli buffer.
Laemmli buffer reaction stop and denaturation.
Reactions were terminated by addition of Laemmli sample buffer.
Laemmli sample buffer was added to the final bead pellet.
The proteins are extracted with complete Laemmli buffer.
Ulrich Laemmli made a crucial contribution to a method for separating proteins by electrophoresis.
Cells pellets are dissolved in Laemmli buffer.
The protein fractions are denatured in Laemmli sample buffer and electrophoresed on SDS-Polyacrylamide gels.
Cells were lysed directly in the wells using reducing Laemmli buffer.
The sample buffer used for the Laemmli method above was used for this method.
The cells were lysed and boiled in two times concentrated Laemmli buffer.
Lysis was done in Laemmli Sample buffer.
Whole proteins were directly extracted using IX Laemmli buffer.
Cells are lysed in Laemmli sample buffer and subjected to SDS-PAGE.
See also
Reaction was stopped with Laemmli buffer.
We mixed aliquots with Laemmli loading buffer and electrophoresed them on SDS-PAGE.
Immunoblotting Analysis Cells were washed with PBS and directly lysed in laemmli buffer.
In accordance with the Laemmli method Nature, vol.
Thirty hours after transfection, the cells were lysed in Laemmli buffer.
The pellets were resuspended in Laemmli buffer and loaded for SDS-PAGE analysis.
The phosphorylation reaction was terminated after 30 minutes by addition of Laemmli sample buffer.
The pellet was dissolved in Laemmli buffer and subjected to SDS-PAGE under nonreducing conditions.
The reaction was stopped after 15 min at room temperature with boiling Laemmli buffer.
The pellet was suspended in Laemmli sample buffer and adjusted to 1.4 mg SDS / mg protein.
Following the induction, the bacterial cells were subjected to lysis by Laemmli sample buffer.
To the reaction tubes was added 40 microliters of Laemmli 2X sample dilution buffer containing B-mercaptoethanol.
Samples were run on polyacrylamide slab gels ( at the appropriate concentration ) using the Laemmli method.
The remaining protein lysates are diluted 1:1 with laemmli sample buffer containing B-mercaptoethanol.
SDS-PAGE was carried out by the method of Laemmli.
The precipitate had 20 µL of 2 x Laemmli sample buffer added to it.
All gels in the Examples are also denaturing SDS-polyacrylamide gels according to Laemmli.
Proteins were electrophoretically separated on 1 % agarose gels in Laemmli sample buffer under reducing conditions.
The SDS-polyacrylamide gel electrophoresis was carried out according to the method described in Laemmli U. K.
Briefly, the buffer system of Laemmli was used to prepare 12 % polyacrylamide gels.
For Western blot analysis, cells were lysed in Laemmli buffer.
The electrophoresis was performed in a Laemmli system using a 10 % separating gel and a 4 % stacking gel.
SDS-PAGE was performed according to the Laemmli method.
Concentrated samples were added 5 x Laemmli sample buffer and were heated to 95°C for 5 minutes.
SDS-PAGE was performed according to the method of Laemmli UK.
Proteins were eluted from the resins by boiling in Laemmli buffer supplemented with 2.5 % B-mercaptoethanol.
Finally, the matrix was treated with 40 µl of non reducing Laemmli buffer.
Add laemmli sample buffer ( LSB ) to samples and controls ( see LSB directions ).
In these experiments, living cells were solubilized with boiling Laemmli buffer to block protease activity.
One-dimensional electrophoresis was carried out on 13 % polyacrylamide gels according to the method of Laemmli.
Purified antigen was separated on 4 % gel by the Laemmli method in the usual way.
Immunoprecipitated proteins were electrophoresed on 10 % polyacrylamide gels according to the method of Laemmli ( 30 ).
Samples were diluted into Laemmli sample buffer and loaded onto 7.5 % SDS-PAGE gel.
The reaction stopped by addition of 4X Laemmli sample buffer.
They are boiled for 5 minutes, then analyzed by SDS-PAGE electrophoresis according to the Laemmli method.
