Examples of 'lightcycler' in a sentence
Meaning of "lightcycler"
LightCycler is a noun that represents a specific brand or type of rapid thermal cycler used in molecular biology. It is often employed in PCR (polymerase chain reaction) techniques to amplify and detect DNA samples quickly and with high precision
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- A device used to conduct polymerase chain reactions
How to use "lightcycler" in a sentence
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lightcycler
Amplifications were performed in LightCycler capillaries.
The LightCycler is a combined apparatus of Thermocycler and flourimeter.
All capillaries are then sealed and placed in the LightCycler apparatus.
A very important feature of the LightCycler instrument is the color compensation software.
Hybridisation mixtures were subjected to the following temperature regime in the LightCycler.
The LightCycler principle is very similar to that of the Thermocycler.
Very much preferred is a kit for amplifying a target nucleic acid using the LightCycler.
Available commercially for research purposes is the LightCycler TeIoTAGGG hTERT quantification kit Cat.
The standard samples are amplified in separate capillaries but within the same LightCycler run.
Available commercially for research purposes is the LightCycler TeloTAGGG hTERT quantification kit Cat.
HyBeacon probes were hybridised to perfectly matched and mismatched oligonucleotides and analysed in a LightCycler.
Roche expects to begin accepting orders for LightCycler Nano instruments in early November.
Confirmation of PCR amplification reaction using LightCycler.
LightCycler Green and other dyes can be used for genotyping and scanning applications.
Suitable probe systems are for example Taqman probes or LightCycler probes.
See also
However, at present the LightCycler may only excite fluorescein.
The fluorescence signals were detected and calculated with the Lightcycler software.
Data were analyzed with the LightCycler software using the " proportional second derivative maximum " option.
The detection method is suitable for use on the Roche LightCycler platform.
In case in of Lightcycler probes, each probe system consists of two probes acting cooperatively.
The efficiency of the knock-out was determined by analyzing mRNA levels using lightcycler quantification.
Fig . 6 demonstrates that LightCycler Green can be used to scan for sequence variants.
The reaction mixture was then introduced into a capillary tube, which was introduced into the LightCycler.
Suitable probe systems for analysis are Taqman probe, LightCycler probes or Scorpion primers.
LightCycler Green was used for fluorescent monitoring of the melting transition between double-and single-stranded products.
PCR with fluorescence monitoring was performed in 10 µl volumes in the LightCycler instrument.
The results calculated by the Lightcycler software are shown in Figure 2.
Rapid cycle PCR with fluorescence monitoring was performed in 10 µl volumes using the LightCycler instrument.
LightCycler Green was included in the PCR reaction at 10 µM unless otherwise indicated.
Homogeneous amplification and detection of targets may be performed using a LightCycler instrument ( Roche Diagnostics ).
LightCycler probes utilize the technique of fluorescence resonance energy transfer ( FRET ).
Commercially available systems such as Taqman, Lightcycler or others.
Amplification was performed in a LightCycler instrument ( Roche Applied Science ) according to the following thermocycling protocol,.
Signal change is observed in the F1 channel of the LightCycler apparatus.
Version 3.01 of the LightCycler software required the user to set the fluorescence gain manually.
The quantitative real-time PCR was performed using the LightCycler System.
The PCRs were performed with the LightCycler system ( Roche Molecular System Inc ).
The PCR reaction mixture of 7 wherein the cyanine dye is LightCycler Green.
Melt peak data obtained only with LightCycler software version 3.5 have been included in table 3.
Amplificates were then detected by means of Lightcycler ( dual ) probes.
QPCR was run on the LightCycler 480 system with previously published assays targeting CHY and CH1.
Melt curves were all generated using a single LightCycler instrument ( LC2 ).
The LightCycler ® Instrument.
All assays ( C3 and methylation specific ) were performed using the Lightcycler platform.
All melt peaks were derived using a single LightCycler ( LC2 ) to avoid instrument-to-instrument variability.
The amplification and melting were performed on the Roche Lightcycler LC480.
After centrifugation, place in LightCycler LC480 with the following cycling conditions and begin the assay,.
Quantitative HyBeacon PCR analyses were performed with LightCycler and ABI 7700 instruments.
Lightcycler instrument ( Roche ) is a real-time PCR cycler.
QPCR was performed on the LightCycler 480 system.
