Examples of 'lithium acetate' in a sentence
Meaning of "lithium acetate"
lithium acetate: Lithium acetate is a chemical compound used in various applications such as molecular biology, particularly in the purification and precipitation of DNA
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- the lithium salt of acetic acid; used as a buffer in electrophoresis
How to use "lithium acetate" in a sentence
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lithium acetate
Preferred salts are sodium acetate and lithium acetate.
Lithium acetate was dissolved in water prior to addition to the resin.
Transformation was carried out by the lithium acetate method.
A mixture of lithium acetate and ammonium formate also yielded excellent flat compressive strength curves.
Yeast transformation is carried out by lithium acetate method.
The methods involve long PEG and lithium acetate incubation times and transformation frequencies are low.
Yeast transformation was carried out by lithium acetate method.
To maintain lithium acetate in such concentrations, a lithium compound may be added.
Control experiment f was carried out in the absence of lithium acetate.
Millimols of lithium acetate.
Particularly preferred lithium promoters are lithium hydroxide and lithium acetate.
A process according to claim 5 wherein lithium acetate is used as the catalyst.
As such, the lithium acetate concentration can be more effectively maintained and / or controlled.
The alkali metal compound may include lithium acetate.
There are small amounts of entrained lithium acetate that require periodic, intermittent replacements.
See also
Yeast transformation was performed using the lithium acetate method.
However, when Lg is bromo, lithium acetate dihydrate is the preferred base.
All yeast transformations were performed using the standard lithium acetate method.
A lithium acetate transformation procedure was done according to the manufacturer 's instructions ( Matchmaker Two-Hybrid system, Clontech ).
A linear increase of reaction rates correlated to increasing lithium acetate concentrations was observed.
Lithium acetate ( CH3COOLi ) is a salt of lithium and acetic acid.
Yeast transformation Yeast transformation was performed using the lithium acetate method.
Example 25 was repeated, the lithium acetate being replaced by 17 mmols of magnesium acetate tetrahydrate.
Most preferably, the salt is lithium iodide or lithium acetate.
The source of lithium acetate used in this example was in the dihydrated Li [ CH3CO2 ].
The process of claim 2 wherein said lithium salt is lithium acetate.
The transformation method used is the lithium acetate method described by Schiestl and Gietz ( 1989 ).
The process of claim 4 wherein said alkali metal salt is lithium acetate.
Yeast cells may be transformed, for example, using the lithium acetate transformation method or electroporation.
The process according to claim 2 wherein the salt is lithium iodide or lithium acetate.
Example 1 was repeated, but with 40 mmols of lithium acetate being added to the reaction mass.
In the examples that follow, transformation of yeast cells was performed with lithium acetate.
As the basic compound, potassium acetate, sodium acetate and lithium acetate are particularly preferred.
Examples of such a method include an electroporation method, spheroplast method and lithium acetate method.
The presentation also includes a step-by-step protocol for the lithium acetate method of yeast transformation.
The process according to claim 9, wherein said salt comprises lithium iodide or lithium acetate.
In illustrative embodiments, such transformation protocols include electroporation, lithium acetate methods, and use of spheroplasts.
Transformation-competent cells were prepared by resuspension of S. cerevisiae cells in 100 mM lithium acetate.
A preferred general method of transformation is the lithium acetate procedure of Ito et al ., above.
A conventional beer yeast was transformed with the pAX43KL according to the lithium acetate method.
The foam of claim 1, wherein the lithium salt comprises lithium acetate and / or lithium stearate.
A process according to claim 1 or 2, wherein the lithium salt is lithium acetate.
Method 1, DNA precipitation with lithium acetate.
Particularly preferred are iodide or acetate salts, e.g., lithium iodide or lithium acetate.
Transformation of yeast is performed by known methods, e.g. lithium acetate method.
In particular, yeast cells were transformed with the MEFA expression plasmid using a lithium acetate protocol.
The process of any of Claims 4-8 wherein the lithium salt is lithium acetate.
Preparation and transformation of yeast competent ( CLONTECH, Yeast Protocols Handbook ) by lithium acetate ( LiAc ) method.
The plasmids described above were used to transform S. cerevisiae BY4741 using standard lithium acetate procedures.
Alternatively, the RNA was precipitated using 2 M lithium acetate.
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