Examples of 'loading buffer' in a sentence
Meaning of "loading buffer"
loading buffer - A section of memory used to temporarily store data while moving it from one location to another, often in the context of computing or technology
How to use "loading buffer" in a sentence
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loading buffer
Cells were lysed in gel loading buffer and collected.
The loading buffer was removed from each well.
The reaction was stopped by adding an isoelectrofocusing loading buffer.
In certain embodiments a loading buffer can comprise a divalent cation.
Equal amount of the urine was loaded using the loading buffer.
Discard the loading buffer and let the plate dry.
Preparation of the loading buffer.
Often the loading buffer and elution buffer will be the same.
This low salt concentration also applies to the loading buffer.
Samples were diluted in gel loading buffer if signal intensities were too great.
The column was washed with a potassium phosphate solution in a loading buffer.
Five volumes of sterile loading buffer were then passed through the column.
The resin is generally equilibrated with multiple volumes of anion loading buffer.
The loading samples are prepared in the loading buffer free of reducing agent.
Loading buffer is the buffer used to load the composition comprising DsbA onto a chromatography material.
See also
Test compounds were added to the cells with the oxonol loading buffer.
The column is washed with loading buffer and then eluted with a salt gradient.
The feed was cell culture supernatant adjusted to different loading buffer conditions.
The samples are applied to loading buffer and DNA fragments are separated.
HEPES may also be used as the loading buffer.
Typically, the loading buffer is used for reequilibrating.
Cells lysate was made in situ by adding a SDS loading buffer to the culture plates.
The loading buffer may comprise sodium chloride, sodium acetate, or a mixture thereof.
Another buffer that is typically employed during Protein A chromatography is a loading buffer.
In some embodiments, the loading buffer is a sodium chloride buffer.
Reaction products were resuspended in 3 µl loading buffer.
In one embodiment, the loading buffer contains sodium chloride.
The concentrated supernatant was diafiltered against 4 volumes of loading buffer.
In some embodiments, the loading buffer is a sodium acetate buffer.
The bound proteins were eluted from the beads by boiling in SDS-gel loading buffer.
In another embodiment, the loading buffer contains sodium chloride.
At the time points indicated, the reaction was stopped by addition of SDS-sample loading buffer.
In one embodiment, the loading buffer contains sodium phosphate.
The nuclear pellets were lysed in SDS-PAGE sample loading buffer.
In one embodiment, the loading buffer does not contain sodium chloride.
The pellet was re-suspended in an equal amount of SDS-PAGE protein loading buffer.
The change from loading buffer to intermediate buffer may be step-wise or gradual as desired.
Before loading on the gel 2 µl loading buffer were added.
The Flue3 loading buffer was prepared as follows.
In some cases, the wash buffer and loading buffer may be the same.
The Fluo3 loading buffer was prepared as follows.
In this case, typically, the wash buffer and the loading buffer are be the same.
A one-tenth volume of gel loading buffer was added and the sample loaded onto the gel.
Elute proteins from the solid support using low-pH or SDS sample loading buffer.
Typically for each chromatographic step, one loading buffer and one elution buffer is needed.
The pellet is washed in said carbonate buffer and resuspended in SDS-PAGE loading buffer.
Twenty µl of the 2X dye loading buffer was then added to each well.
First, the anion-exchange column of the FPIC system was equilibrated with the loading buffer.
All samples were boiled in SDS-PAGE loading buffer prior to electrophoresis.
These fractions were dried down in a Speedvac and re-suspended in HPLC loading buffer.
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And we began loading rods an hour ago
Loading of cassettes via special crane technology
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
