Examples of 'loxp' in a sentence
Meaning of "loxp"
loxp (noun): In genetics, loxP refers to a specific short DNA sequence that serves as a site for the Cre recombinase enzyme. This sequence is commonly used in genetic engineering to manipulate DNA sequences in a controlled and reversible manner
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- A DNA site on the phage P1 genome, with sequence "ATAACTTCGTATANNNTANNNTATACGAAGTTAT" used in genetic engineering.
How to use "loxp" in a sentence
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loxp
None of the loxP x loxP seedlings rooted on hygromycin.
Implementation of multiple loxP site pairs.
The loxP sites are indicated by arrowheads.
It recognizes and binds to specific sites called loxP.
Usually loxP sites come in pairs for genetic manipulation.
This expression cassette is flanked by a LoxP inverted repeat.
All progeny receive the loxP construction from the homozygous parent.
In a most preferred embodiment recombination is at a loxP site or a flp site.
A loxP site includes native loxP sequences as well as modified loxP sites.
The two exemplary recombination sites are attP and loxP.
This enzyme is able to recombine the two loxP sites flanking the kanMX resistance gene.
Said activation is preferably achieved by removal of a stop sequence flanked by loxP sites.
All seven loxP x WT control progeny tested were sensitive to kanamycin.
This is done by introducing short sequences called loxP sites around the gene.
The loxP and FRT sites are recognition sites for the Cre and Flp recombinases.
See also
This double expression cassette was the cloned into an integrative loxP vector.
A Cre recombinase targets the loxP sites for recombination.
In one specific aspect the approach utilizes three heterospecific and incompatible loxP sites.
The sequences of the loxP and FRT sites are shown in SEQ.
The CRE enzyme is a recombinase which specifically recognizes LoxP sites.
Exons are in bold . loxP sequences are highlighted in grey.
The recombination sequences to which the Cre recombinase binds are named loxP.
The system consists of a recombination site designated loxP and a recombinase designated Cre.
In brief, loxP sequences are provided on each side of each selection marker.
Cre recombinase has the capacity to split DNA regions flanked by loxP type sequences.
A LoxP sequence is then introduced on either side of the adenoviral genome, in parallel orientation.
The asymmetric target site has a certain homology to the symmetric loxP site recognized by Cre.
L9 is a homozygous loxP plant derived from a primary transformant not used in previous experiments.
The two recombination sites are attP and loxP on the Vector Donor.
The orientation of the loxP - polyA - loxP HindIII insert was determined by digestion with BamHI.
Preferably ; the lox sites are loxP sites.
The forward primer aditionally had a loxP site, an attB site and a PvuI restriction site.
In a specific embodiment, the recombinase recognition site is a loxP site.
Figure 2 shows integration and subsequent removal of a loxP flanked pluripotency determining factor transgene.
In some embodiments, the two lox sites are both loxP sites.
This cassette contains a loxP site and a bacterial / mammalian selectable marker, such as hygromycin resistance.
Preferably, there are at least two loxP sites in a locus.
One clone recombined in loxp shows a 5 kbp band resulting from a rearrangement of the pMCl-Cre plasmid.
Cre-mediated in vivo recombination via the LoxP recognition sites.
Escherichia coli LoxP recombination site, recognized by a Cre recombinase enzyme.
As mentioned, the bipartite selection cassette is flanked by LoxP recombination sequences.
This released the LoxP sandwiched Ura3 gene from the genome.
Re-transformed plants were analyzed to demonstrate site-specific recombination at the loxP sites.
Preferably, said sequences are LoxP sequences of the Cre recombinase.
A method according to claim 7 wherein three heterospecific and incompatible loxP sites are used.
DNA sequences found between two loxP sites are said to be " floxed.
Cre1 is a recombinase that removes the sequences located between the loxP sequences.
One example of such a system is the cre / loxP recombinase system of bacteriophage P1.
The method of claim 5, wherein said site preselected for recombination is a loxP site.
The Cre - loxP strategy was used also in connection with inducible promoters.
