Examples of 'lysis buffer' in a sentence
Meaning of "lysis buffer"
Lysis buffer is a solution used in biological and biochemical experiments to break open cells and release their contents. It typically contains detergents, salts, and enzymes that help disrupt the cell membrane and break down cellular components
How to use "lysis buffer" in a sentence
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lysis buffer
The lysis buffer comprises guanidine thiocyanate.
Samples were exposed to lysis buffer as indicated above.
A lysis buffer containing protease inhibitors is added.
The pellet is resuspended in the lysis buffer.
Importantly a lysis buffer is not employed.
Purification of crude virus solution in lysis buffer.
Samples are prepared using lysis buffer as discussed above.
The immunoprecipitates were washed extensively with lysis buffer.
Pellets are washed once in lysis buffer and centrifuged.
Proper processing includes formulating an appropriate lysis buffer.
A preferred lysis buffer includes a detergent.
Background was the count of lysis buffer only.
Cells were lysed with lysis buffer supplemented with protease inhibitors.
Lyse the cells with lysis buffer.
The lysis buffer from each well was transferred to an individual tube.
See also
Cells are resuspended and incubated in lysis buffer.
A universally applicable lysis buffer is thus a broadly usable lysis buffer.
It may be the most widely used lysis buffer.
Cells were lysed on ice in lysis buffer and protein concentration determined.
Bound beads were washed three times in lysis buffer.
This is only possible if the lysis buffer comprises a chaotropic reagent.
A second container may comprise a stool lysis buffer.
Frozen tissues were lysed with cell lysis buffer containing protease inhibitor.
Afterwards cells were rinsed and lysed in lysis buffer.
Further the lysis buffer may comprise at least one nucleophilic reagent.
Beads were washed with lysis buffer.
Lysis buffer is included as a background control when replacing the tissue samples.
Negative controls consist of amplification of lysis buffer extracts.
The pH of the lysis buffer described above is not restricted to specific pH values.
Cells were harvested and lysed with lysis buffer.
The lysis buffer can be any buffer known for cell lysis.
Incubation was stopped by addition of lysis buffer.
The lysis buffer according to the present embodiments was also included as a control.
The column was washed extensively with the lysis buffer.
Twenty milliliters of lysis buffer was added to the cell pellets and vortexed.
Contacting the fixated sample of step a with a lysis buffer.
The pellet is resuspended in a volume of lysis buffer equal to the supernatant volume.
Equal concentrations of cell lysates were prepared with lysis buffer.
The tissue was treated with lysis buffer and homogenized.
The remaining adherent cells were gently scrapped in lysis buffer.
These inhibitors were added fresh to the lysis buffer preparation at time of use.
The collected cells were dissolved in an appropriate volume of lysis buffer.
The bead was washed with lysis buffer four times.
This epidermis was manually ground in a glass homogenizer in TriReagent lysis buffer.
Frozen brain punches were transferred into lysis buffer and immediately homogenized.
Also disclosed is that the sample preparation reagent is a lysis buffer.
Values from wells containing lysis buffer and substrate only were subtracted as background.
The membrane pellet was solubilized in the lysis buffer by sonication.
Beads were washed with lysis buffer before elution and reduction prior to immunoblotting.
The extraction was repeated with the same lysis buffer.
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Examples of using Lysis
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Lysis from without could not be demonstrated
It may also lead to a lysis of certain particles
The lysis buffer comprises guanidine thiocyanate
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
