Examples of 'mercaptoethanol' in a sentence
Meaning of "mercaptoethanol"
mercaptoethanol (noun) - a chemical compound commonly used in biological and chemical research as a reducing agent or to protect against oxidation
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- A compound of ethanol with a thiol group, often specifically 2-mercaptoethanol, which is used in various organic chemistry applications including protein denaturation
How to use "mercaptoethanol" in a sentence
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mercaptoethanol
Other nucleophiles include thiols such as mercaptoethanol.
The sulfur in the mercaptoethanol moiety is oxidized to sulfone.
Samples were prepared by boiling in sample buffer with mercaptoethanol.
Mercaptoethanol is used as a chain transfer agent.
Examples of the reducing agent are mercaptoethanol and mercaptoethylamine.
An example of a hydroxyl functional chain transfer agent is mercaptoethanol.
Mercaptoethanol reduction of an antibody yields individual heavy and light chains.
Unreacted allyl groups are readily blocked using mercaptoethanol.
Reduction with mercaptoethanol makes the biological activity drop practically to zero.
Samples were reduced with mercaptoethanol.
Treatment with mercaptoethanol and rabbit liver esterase was used to deblock the peptide.
An example of a chain transfer agent having hydroxyl groups is mercaptoethanol.
The derivatised tAK is reduced by reaction with mercaptoethanol to yield a reactive sulfhydryl group.
The proteins were treated with sample buffers with or without mercaptoethanol.
DTT or mercaptoethanol are adjusted to achieve this goal.
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The solid was washed with water until no odor of mercaptoethanol remained.
Pretreatment with mercaptoethanol was as for the SPR glass slides.
Excess maleimide groups can be quenched by adding free thiol reagents such as mercaptoethanol.
Similar competition reactions between the dithiols and mercaptoethanol were used in the potentiometric study.
Mercaptoethanol is used in some RNA isolation procedures to eliminate ribonuclease released during cell lysis.
Chain transfer agents such as isopropanol and mercaptoethanol may also be employed.
Mercaptoethanol was omitted for non-reduced samples.
A preferred mercapto compound is mercapto alcohol, such as mercaptoethanol.
Preferably, mercaptoethanol and dithiothreitol are used.
Mercapto compounds are preferably used as regulators, mercaptoethanol being particularly preferred.
Mercaptoethanol serves as a cysteine model containing a thiol group and is abbreviated Cys-SH.
The latter was reacted with sodium mercaptoethanol to afford sulfinate salt 1.
After the reaction, the remaining maleimide groups are blocked with mercaptoethanol.
Mercaptoethanol inhibited the initiation as well as the further development of young, unpigmented sclerotia.
Washed platelets were solubilized in SDS-sample buffer with and without mercaptoethanol.
Reaction 2 represents the formation of poly ( ethylene glycol ) mercaptoethanol by reaction of the mesylate with mercaptoethanol.
The pharmaceutical composition of claim 28 wherein the alcohol containing a thiol moiety is mercaptoethanol.
Mercaptoethanol was removed by dialysis for 12 hours and denatured ADH was recovered by freeze drying.
This process eliminates the need for thiol-reducing agents such as mercaptoethanol or glutathione.
Safety note, ß - mercaptoethanol is toxic, dispense in a fume hood and wear appropriate clothing.
The thiol group is introduced by the reaction product of dimethyl-m-isopropenyl benzyl isocyanate and mercaptoethanol.
Finally, the hydrogel was suspended in the mercaptoethanol containing buffer and incubated for 3 h at RT.
Exhaustively extracting the resultant hydrogel with water to remove all residual urea, thiourea, and mercaptoethanol.
Catalyst was added followed by mercaptoethanol ( portionwise ) . 5 molar % of catalyst were used.
This enzyme has at least one effector, Mercaptoethanol.
If what we are smelling was natural gas, there would be more than one peak . Mercaptoethanol.
Examples of other reducing agents include DTT ( dithiothreitol ), mercaptoethanol and mercaptoethylamine.
The reaction was then quenched by the addition of 4 µl of 10 mM mercaptoethanol.
This reagent is stable during 1 month but 50 µl of mercaptoethanol is added every 2 days.
When A is the sulfur atom, the compound I is advantageously mercaptoethanol.
Adhesives made in ethyl acetate included 0.024 parts of mercaptoethanol as a chain transfer agent.
Briefly, cells were collected by centrifugation and lysed in a solution of 4M guanidinium thiocyanate containing 4 % mercaptoethanol.
Then the unreacted maleiimidyl residues were inactivated by adding 0.005 ml mercaptoethanol to each reaction mixture.
Nonlimiting list of transfer agents is given herein, isopropyl alcohol, sodium hypophosphite, mercaptoethanol.
Stabilizing agents such as dithiothreitol ( DTT, Cleland 's reagent ) or mercaptoethanol may also be included.
