Examples of 'microsequencing' in a sentence
Meaning of "microsequencing"
microsequencing (verb) - a process of determining the order of nucleotides or amino acids in a molecule with great precision, often used in genetics and biochemistry
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- The sequencing of very small amounts of protein or polypeptides
How to use "microsequencing" in a sentence
Basic
Advanced
microsequencing
In practice this microsequencing analysis is performed as follows.
A highly preferred method is the microsequencing technique.
Microsequencing is a preferred method of genotyping alleles.
Purified protein is used for protein microsequencing.
The microsequencing reaction performed as follows.
Cys residues can not be identified by protein microsequencing.
Peptides identified by microsequencing are shown in underline.
Each band was excised and subjected to protein microsequencing.
Standard or microsequencing methods can be applied to protein isolated by immunoprecipitation.
Eight peaks were subjected to microsequencing.
Microsequencing of this material reveals a unique terminal amino acid sequence.
The proteins in these bands were identified by tandem mass spectrometry microsequencing.
Microsequencing is achieved by the established microsequencing method or by developments or derivatives thereof.
The proteins thus purified are analysed by microsequencing the amino acids.
Microsequencing may be achieved by the established microsequencing method or by developments or derivatives thereof.
See also
Different approaches can be used to detect the nucleotide added to the microsequencing primer.
Microsequencing was performed by Edman degradation in an automated sequencer.
The underlined nucleotides correspond to the possible nucleotide positions detected by the microsequencing assay.
Microsequencing of the purified r-IFNt was carried out.
Genotyping of individual DNA samples was performed using a microsequencing procedure.
The microsequencing reaction was performed as follows,.
The results were confirmed by microsequencing and bioassay,.
Spot microsequencing could precisely identify cross-reactive antigens.
The amino acid sequence 's accuracy was confirmed by microsequencing.
Microsequencing methods determine the identity of only a single nucleotide at a “ predetermined ” site.
Preferably, genotyping typing is performed using the microsequencing technique.
Several peptides were subjected to microsequencing and yielded the following oligopeptide sequences,.
In a preferred embodiment, wherein said determining is performed by a microsequencing assay.
Microsequencing primers were designed for each of the biallelic markers, as previously described.
Alternative methods include several solid-phase microsequencing techniques.
Microsequencing revealed an amino-terminal sequence containing no significant homology to any known protein.
The purified material was then subjected to gas-phase microsequencing.
For ' large ' proteins gas phase microsequencing was used to confirm the correct sequence.
Method according to any of claims 1 or 2 wherein said determining is performed by a microsequencing assay.
As yet another alternative microsequencing procedure, Nyren et al.
This region of the gel was cut out and subjected to gas-phase microsequencing.
Preferred microsequencing primers are described in Table 4.
In some embodiments, the amplification primers or microsequencing primers may be labeled.
Microsequencing of the internal regions of Tbp2 was therefore affirmed as reported above in 1C.
For example, in some embodiments, the amplification primers or microsequencing primers may be biotinylated.
Microsequencing of the purified material reveals a unique N-terminal amino acid sequence.
These standard methods include affinity purification, microsequencing and cDNA databank searches.
The gas-phase microsequencing demonstrated unambiguously a single N-terminal amino acid sequence.
In one such embodiment, the subsequent reaction is a genotyping reaction, such as a microsequencing reaction.
Example 2 describes amino acid microsequencing of the purified human plasma PAF-AH.
The sequence yielded an ORF, containing most of the peptide sequences obtained by microsequencing.
The primers used in the microsequencing reactions may include the primers listed in Table 4.
DNA sequences may, for example, be identified by direct microsequencing of the purified DNA.
Microsequencing was carried out for each individual DNA sample described in Example 11.
According to the invention, the primers used in microsequencing are detailed in Table 4.
