Examples of 'minigene' in a sentence
Meaning of "minigene"
A short segment of DNA that is used in research to study specific genes or genetic elements, typically smaller than a full-length gene. It is often manipulated and inserted into cells to observe the effects or functions of the gene
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- A gene segment that codes for a variable portion of the protein immunoglobulin.
How to use "minigene" in a sentence
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minigene
This is a minigene in its most basic sense.
The rAAV described herein also comprise a minigene.
The following are examples of minigene use in various diseases.
Such a minigene can be inserted into an appropriate expression vector.
T cell responses to minigene vaccination.
Helper virus may optionally contain a second reporter minigene.
Minigene plasmid constructs.
Herein the one or more amyloidogenic proteins can be encoded by a minigene.
A minigene usually lacks all of the introns of the genomic gene.
A cDNA encoding a gene product may also be used to construct a minigene.
The presence of such epitopes in a minigene could significantly affect minigene performance.
The minigene is used to deliver the antigen to the immune system of a subject.
Additional vector modifications may be desired to optimize minigene expression and immunogenicity.
Then a minigene capable of expressing that fusión enzyme is constructed using known techniques.
Additional vector modifications may be desired to optimize minigene expression end immunogenicity.
See also
A recombinant hpAP minigene was designed and inserted into the BglII site of pAd.
The nucleic acid encoding the epitopes are assembled in a minigene according to standard techniques.
Therefore release of the minigene from the plasmid was accomplished by digestion with NheI.
The inclusion of mRNA stabilization sequences can also be considered for increasing minigene expression.
A minigene of the disclosure can be packaged for use in the clinical and research laboratories.
A very large number of different epitope arrangements can be considered in designing a particular minigene construct.
The GS minigene was arranged to transcribe in the same direction as procollagenase.
A preferred means of administering nucleic acids encoding the peptides uses minigene constructs encoding multiple epitopes.
The sequences of the minigene constructs were verified by Sanger sequencing.
In vivo immunogenicity is a second approach for functional testing of minigene DNA formulations.
In this new vector the LacZ minigene is replaced with the CFTR minigene.
In vivo immunoganicity Is a second approach for functional testing of minigene DNA formulations.
A minigene expression plasmid typically includes multiple CTL and HTL peptide epitopes.
The linear arrangement of this minigene includes,.
The GS minigene is present as a selectable marker for use following CHO cell transfections.
Production of transformed cells, minigene constructs.
Minigene Vaccines A number of different approaches are available which allow simultaneous delivery of multiple epitopes.
For example, a minigene is prepared as follows.
In still another embodiment, a modified adenoviral particle carrying a minigene is delivered to a subject.
Alternatively, the minigene may be provided in an expression vector.
To optimize expression and / or immunogenicity, additional elements can be incorporated into the minigene design.
In the context of minigene vaccines, the controversy has been renewed.
For instance, the vector may contain the rAAV construct comprising the minigene.
If specific trends are detected, second generation minigene vaccines are designed based on these trends.
A minigene comprising AAV inverted terminal repeats ; and.
Expression of the glutamine synthetase minigene is driven by the SV40 late promoter.
Two splicing mutations of CASQ2 have been validated using a splicing minigene assay.
Somatic B-cell minigene transfer has been described in detail elsewhere.
Southern hybridization indicates cell lines contain the CYP7 minigene construct.
Optionally the minigene can contain the promoter region of CYP7.
In reaction II, an analogous fused coding region is obtained from the a minigene described hereinabove.
A fusión Fc construct or minigene comprise a polynucleotide encoding a polypeptide / Fc fusión polypeptide.
By comparison, only two such junctional motifs are present in the HCV1 minigene assortment.
Expression of dystrophin minigene may be detected by immunofluorescent staining and immunoblotting ( Western blotting ).
Southern hybridization indicates which founder mice retain the CYP7 minigene construct.
