Examples of 'miniprep' in a sentence
Meaning of "miniprep"
Miniprep is a laboratory technique that involves the rapid extraction of small quantities of purified DNA
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- A rapid, small-scale isolation of plasmid DNA from bacteria.
- To perform a miniprep procedure.
How to use "miniprep" in a sentence
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miniprep
Colonies were picked and miniprep DNAs were made.
Then do miniprep and restriction mapping to screen for desired product.
Rapid isolation of miniprep.
Miniprep colonies were prepared and the plasmids were sequenced to check the construction.
An aliquot of the culture used for the miniprep is stored as a glycerol stock.
Miniprep kits for plasmid purification were from Quiagen.
Plasmid DNA was recovered by using plasmid miniprep kit.
Wizard plasmid miniprep kits and RNasin were from Promega.
Template DNA is then prepared using a plasmid miniprep.
Candidate clones were grown and miniprep plasmid DNA was prepared.
Miniprep colonies were prepared and the plasmid DNA was sequenced.
DNA was isolated using a miniprep procedure.
Miniprep DNA obtained from these clones was then used for sequencing.
This is an example of initial screening data miniprep DNA.
Miniprep DNA from four out of eight pools was positive when tested for expression.
See also
Perform restriction enzyme digestion analysis of the miniprep DNA.
The miniprep DNA was screened by PCR and the sequence was verified.
Total DNA was isolated using a slightly modified miniprep protocol.
Transformants is screened by DNA miniprep and the desired constructs are confirmed by DNA sequencing.
Plasmid DNA was isolated using the following alkaline miniprep protocol.
The remainder of the miniprep DNA was used to transfect two CHO cell lines.
Plasmid DNA was isolated using a standard alkaline lysis miniprep protocol.
Four transformant colonies were picked and miniprep DNA was prepared using Qiagen Miniprep Kit.
Colonies of all assembled plasmids were initially screened by restriction digestion of miniprep DNA.
Kanamycin resistant clones were grown and miniprep plasmid DNA was prepared.
The plasmids of those cultures were extracted using the Zymoprep Yeast Plasmid Miniprep kit.
Glycerol stock and miniprep for expression Example 2.
Plasmid DNA for each of these vectors was then obtained by a miniprep method.
More specifically, miniprep DNAs are digested with the diagnostic restriction enzyme, Bam HI.
The presence and identity of the plasmids was confirmed by DNA miniprep and restriction digest.
The C. difficile miniprep DNA was used as a template for PCR sequencing to verify clone integrity.
Glycerol stock unique LC combinations and miniprep DNAs.
Following electrophoresis of the digested miniprep DNA, different DNA fragment patterns could be observed.
Colonies were grown in solution and DNA was isolated using Qiagen miniprep columns.
From this culture a miniprep of plasmid DNA was MADE using the Wizard Plus kit ( Promega ).
Clones with inserts were selected by screening miniprep DNAs with Bgl II.
The Qiagen Spin Miniprep Kit was used to make plasmid DNA, following the manufacturer 's instructions.
Plasmid DNA was purified using QIAGEN spin miniprep kit and sequenced.
Briefly, miniprep pET-PBR plasmid dsDNA was used as template.
Random colonies were picked and plasmid DNA purified using Qiagen miniprep kits.
In essence, this is a scaled-up miniprep followed by additional purification.
Plasmid DNA was isolated from defined culture volumes using QIAGEN Miniprep kits.
The resulting volume per miniprep sample should be 50µl.
Count colonies on plates and pick 12 colonies from each transformation reaction for miniprep and sequencing.
Plasmid was isolated using miniprep kit ( Sigma ) on 3 mL cell culture.
B-galactosidase-negative transformants were screened by restriction digestion of alkaline lysate plasmid miniprep DNA.
Plasmid was isolated from 4 colonies using QIAprep Spin Miniprep kit and subjected to sequence analysis.
Plasmid DNA was isolated from the resulting bacterial colonies using a Promega Wizard ™ miniprep kit.
Prepare miniprep DNA using QIAVac 96 kit following manufacture 's protocol.
Glycerol stock unique HC combinations and miniprep plasmid DNA.
