Examples of 'minutes on ice' in a sentence
Meaning of "minutes on ice"
minutes on ice: In ice hockey, refers to the amount of time a player spends on the ice during a game, typically tracked to monitor player performance and fatigue
How to use "minutes on ice" in a sentence
Basic
Advanced
minutes on ice
The coupling reaction was incubated 5 minutes on ice.
After stirring for 20 minutes on ice the solution was let to warm to room temperature.
Antibodies were then added to cell suspensions and incubated for 20 minutes on ice.
Precipitation was allowed to proceed for 10 minutes on ice and thereafter samples were centrifuged.
The cells and the above antibodies were mixed gently and incubated for 10 minutes on ice.
The cells were incubated for approximately 15 minutes on ice to allow for complete lysis.
MIN Minutes on ice - Total number of minutes the goaltender has been on the ice.
Bone marrow cells were lysed in ACK lysis buffer for 7 minutes on ice.
The mixture was left for 10 minutes on ice and centrifuged.
Supernatant was extracted and lysed in ACK lysis buffer for 7 minutes on ice.
The suspensions were incubated for 60 minutes on ice with occasional vortexing.
The suspension is stirred vigorously for approximately 30 minutes on ice.
Cells were incubated for 90 minutes on ice and then washed twice in PBSA.
The mixture is incubated for 30 minutes on ice.
Cells were incubated for 30 minutes on ice and washed twice with FACS buffer.
See also
Samples are incubated for another 30 minutes on ice.
Cells are then stained for 30 minutes on ice or at 4°C protected from light.
Stirring was continued for another 20 minutes on ice.
The mixture was incubated 30 minutes on ice before the tubes were transferred at 30°C.
The mixture was stirred for 30 minutes on ice.
The samples were incubated for 30 minutes on ice with streptavidin-conjugated RPE ( phycoerythrin ).
The microtube was incubated for 30 minutes on ice.
We then incubated the samples for 30 minutes on ice with steptavidin-conjugated RPE ( phycoerythrin ).
The assay was incubated for 30 minutes on ice.
The mixtures were incubated for 60 minutes on ice in the dark.
The refolding solution was mixed and allowed to incubate 10 minutes on ice.
The overall suspension was incubated 30 minutes on ice and sonicated.
Cold receptor-Fv fusión protein is allowed to bind for 40 minutes on ice.
The plate was then incubated for 30 minutes on ice in a dark container.
Compound 1, 2 or wortmannin are incubated with mTOR enzyme for 30 minutes on ice.
The mixture was incubated for 60 minutes on ice.
Cells were incubated with rat or humanised antibody for 30 minutes on ice.
Reactions were incubated for 45 minutes on ice.
The plate with aluminum film is sealed and incubated for 10 minutes on ice.
The cells were lysed for 30 minutes on ice.
Seal the plate with aluminum film and incubate for 10 minutes on ice.
This mixture is allowed to incubate for 30 minutes on ice.
Incubation was again carried out for 30 minutes on ice.
The cell pellets were left to stand for 10 minutes on ice.
Each reaction was carried out for 140 minutes on ice.
The cells were then collected centrifugation and lysed for 15 - 30 minutes on ice.
Following incubation, the cells were washed and fixed with 3 % paraformaldehyde for 15 minutes on ice.
Two volumes 98 % ethanol are added to the extracted supernatant and left for 10 minutes on ice.
Transformation was effected by heat shock at 42 °C for 45 seconds followed by 2 minutes on ice.
The cleanest fractions were pooled and treated with 10 mM TCEP for 20 minutes on ice.
The wash and elution fractions were all treated with 50 mM DTT for 30 minutes on ice.
Then, cells will be washed and permeabilized in Cytofix / Cytoperm ( BD PharMingen ) for 20 minutes on ice.
Elsevier Biomedical Press, Amsterdam of HB101 with the ligated plasmid for 30 minutes on ice.
You'll also be interested in:
Examples of using Minutes
Show more
Just give me a couple minutes to catch my breath
Ten minutes more and we will eat
Just give me five minutes for my article
Examples of using Ice
Show more
Ice machine electronic control in top of door
Preparing the ice machine for storage
Ice may fall from any structure
