Examples of 'ml of fresh' in a sentence

Meaning of "ml of fresh"

ml of fresh: Likely part of a measurement or recipe, indicating milliliters of a fresh substance, such as a liquid or ingredient

How to use "ml of fresh" in a sentence

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ml of fresh
Ml of fresh sweat was introduced into each flask.
The medium was then removed and replaced by 4 ml of fresh medium.
Ml of fresh blood was obtained from donors.
Gram of each extract was placed in a pill box containing 1 ml of fresh sweat.
A volume of 6 ml of fresh medium was added to the release samples.
The transfection mix was incubated for 6h before being replaced by 4 ml of fresh medium.
This washing is repeated with 2 ml of fresh ethanol and the supernatants combined.
The supernatant was aspirated and the cells were resuspended in 50 ml of fresh growth medium.
The medium is decanted off, 2 ml of fresh medium are added and vortexing is repeated.
After overnight incubation the inoculum / medium was replaced with 2 ml of fresh medium.
The medium was removed and 2 ml of fresh medium were added and the vortexing repeated.
The original transfected cells were further incubated after adding 8 ml of fresh medium.
The wash media were discarded and 5 mL of fresh infection media with drug was added.
Then 3 ml of fresh medium was added and the cells were incubated for five days.
The assay tube was then filled with 5 ml of fresh human blood.

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Add 4 mL of fresh filtered DNPH solution to the volumetric flask.
The separated aqueous portion was extracted twice with 50 ml of fresh methylene chloride.
Ml of fresh TiCl4 were added.
Twenty four hours later medium was replaced with 9 ml of fresh serum free medium.
Then add 50 ml of fresh lemon juice and blend all these ingredients.
Two to four hours after plating the medium was replaced with 5 ml of fresh medium.
The wash buffer was decanted and 30 ml of fresh buffer added and the process repeated.
The media will be removed from the well and replaced with 1 ml of fresh media.
The wash buffer is decanted and 30 ml of fresh buffer added and the process repeated.
The cells are washed by centrifugation and resuspended in 10 mL of fresh medium A.
One to two days later 10 ml of fresh medium were added to cells.
The methanol was removed and replaced with another 600 ml of fresh methanol.
Medium was removed, and 1 ml of fresh medium was added into each well.
The aqueous phase was extracted once with 10 mL of fresh chloroform.
The residue is dissolved in 50 ml of fresh 6 N HCl and is again evaporated to dryness.
After incubation for 2 h, the supernatant was replaced by 2 ml of fresh culture medium.
The polymer was washed with 50 ml of fresh THF and filtered, leaving a grayish-purple powder.
The transfer is completed using 2 mL of fresh dry THF.
Add 9 mL of fresh growth medium ( formula below ).
The supernatant was replaced with 15 ml of fresh PBS and the tubes were retumbled.
The medium was aspirated and the wells were replenished with 0.2 ml of fresh medium.
The medium is then replaced with 50 ml of fresh medium and shaking initiated.
A 50-µl aliquot of the overnight culture was used to inoculate 2 ml of fresh medium.
Then, the culture medium was changed with 1 ml of fresh medium supplemented with 1 % of serum.
This was repeated a third time using 30 mL of stripped organic and 30 mL of fresh acid.
In addition, 25 ml of fresh culture medium is added to the flask.
The methanol was then decanted and 50 mL of fresh methanol added.
Transfer 2.5 ml into 25 ml of fresh broth in an Erlenmeyer flask and shake another day.
Cells were resuspended in 10 ml of fresh media.
Decant fixative, add 5 ml of fresh fixative, and allow to stand 5 minutes.
The mother liquor is decanted off and 200 ml of fresh EtOH is added.
Add 2.0 ml of fresh Color Reagent to all tubes and vortex well.
The syrup was redissolved in 30 ml of fresh methylene chloride.
The cells were precipitated by centrifugation at 1000 rpm and resuspended in 20 mL of fresh media.
The solvent was decanted and 40 ml of fresh pentane was added.

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